CD14 recombinant protein

Recombinant human CD14 protein

Cat. Num. AAA48423

• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CD14; N/A; Recombinant human CD14 protein; monocyte differentiation antigen CD14; CD14 molecule; Myeloid cell-specific leucine-rich glycoprotein; CD14 recombinant protein

• Host: HEK293
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 20-344aa
• Sequence: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSM

• Applicable Applications for CD14 recombinant protein: Bioactivity, SDS-PAGE
• Species: Human
• Tag: His-Tag
• Predicted Nterm: Thr 20
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Bioactivity: Measured by its ability to enhance IL-8 secretion using THP?1 human acute monocytic leukemia cells in the presence of LPS. The ED50 range <= 100 ng/ml.
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human CD14 enhance the IL-8 secretion of the THP?1 human acute monocytic leukemia cells in the presence of LPS. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for CD14 recombinant protein

CD14, also known as monocyte differentiation antigen CD14, is a member of the eucine-rich repeat (LRR) proteins family. It exists in two forms, one anchored to the membrane by a GPI-tail (mCD14), the other a soluble form (sCD14). It is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide(LPS). It acts as a co-receptor (along with TLR4 and MD-2) and helps to detect bacteria in the body by binding LPS, a pathogen-associated molecular pattern (PAMP). Recombinant human CD14, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD14 recombinant protein

Immunology

References

Kelley S.L., et al (2013) J. Immunol. 190:1304-1311.;; Setoguchi M., et al (1989) Biochim. Biophys. Acta 1008:213-222.;;

NCBI and Uniprot Product Information

• NCBI GI #: 291575165
• NCBI GeneID: 929
• NCBI Accession #: NP_001167576.1
• NCBI GenBank Nucleotide #: NM_001174105.1
• UniProt Accession #: P08571; Q53XT5; Q96FR6; Q96L99; Q9UNS3
• Molecular Weight: 40,076 Da
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 molecule
• NCBI Official Symbol: CD14
• NCBI Protein Information: monocyte differentiation antigen CD14
• UniProt Protein Name: Monocyte differentiation antigen CD14
• UniProt Gene Name: CD14
• UniProt Entry Name: CD14_HUMAN