SDS-PAGE
B7-H3 Recombinant Protein | CD276 recombinant protein
B7-H3 Protein, Human, Recombinant (ECD, hFc Tag)
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Purity
>95% as determined by SDS-PAGE >90% as determined by SEC- HPLC.
Synonyms
B7-H3; N/A; B7-H3 Protein, Human, Recombinant (ECD, hFc Tag); Human B7-H3/CD276 Protein (ECD, Fc Tag); 4Ig-B7-H3 Protein, Human; B7-H3 Protein, Human; B7H3 Protein, Human; B7RP-2 Protein, Human; CD276 molecule; CD276 recombinant protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
>90% as determined by SEC- HPLC.
>90% as determined by SEC- HPLC.
Form/Format
Lyophilized from sterile PBS, pH7.4. 5% Trehalose, 5% Mannitol, 0.01% Tween-80
Sequence
Met1-Thr461
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Leu 29
Tag
C-human IgG1-Fc
Protein Construction
A DNA sequence encoding the human CD276 (Q5ZPR3-1)(Met1-Thr461) was expressed with the Fc region of human IgG1 at the C-terminus.
Molecular Mass
The recombinant human CD276 consists 671 amino acids and predicts a molecular mass of 73.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human CD276 is approximately 109.5 kDa due to glycosylation.
Bio Activity
Inmobilized Recombinant Human B7-H3/ CD276 Protein (ECD), Fc Tag) ( MBS8120724) at 2 ug/mL (100 uL / well) can bind Anti-B7 H3 Antibody, IgG1 Kappa, the EC50 is 6.5-20 ng/mL.
Reconstitution
Reconstituted with sterile deionized water to 1.25 mg/ml
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C upon receiving.
Avoid repeated freeze-thaw cycles.
Avoid repeated freeze-thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for CD276 recombinant protein
Background: B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.
Product Categories/Family for CD276 recombinant protein
References
Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 73.25 kDa
NCBI Official Full Name
CD276 antigen isoform a
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
NCBI Protein Information
CD276 antigen; B7 homolog 3; costimulatory molecule
UniProt Protein Name
CD276 antigen
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
UniProt Entry Name
CD276_HUMAN
Similar Products
Product Notes
The CD276 cd276 (Catalog #AAA257968) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Thr46 1. It is sometimes possible for the material contained within the vial of "B7-H3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.