Application Data
(Measured by its binding ability in a functional ELISA. Immobilized CD40 Protein, Mouse, (ECD, His Tag)(50324-M08H) at 2 ug/mL (100 uL/well) can bind CD40 Ligand Protein, Human, (hFc Tag)(10239-H01H), the EC50 of CD40 L-hFc is 70-180 ng/mL.)
CD40 recombinant protein
CD40 Protein, Mouse, Recombinant (ECD, His Tag)
Gene Names
Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
Purity
>95 % as determined by SDS-PAGE.
Synonyms
CD40; N/A; CD40 Protein, Mouse, Recombinant (ECD, His Tag); Mouse CD40/TNFRSF5 Protein (His Tag); CD40 molecule, TNF receptor superfamily member 5; AI326936 Protein, Mouse; Bp50 Protein, Mouse; GP39 Protein, Mouse; HIGM1 Protein, Mouse; IGM Protein, Mouse; IMD3 Protein, Mouse; p50 Protein, Mouse; T-BAM Protein, Mouse; Tnfrsf5 Protein, Mouse; TRAP Protein, Mouse; CD40 recombinant protein
Host
HEK293 Cells
Purity/Purification
>95 % as determined by SDS-PAGE.
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Sequence Positions
185aa
Sequence
Met1-Arg193
Species
Mouse
Tag
C-His
Endotoxin
<1.0EU per ug protein as determined by the LAL method
Predicted N Terminal
Leu20
Protein Construction
|A DNA sequence encoding the mouse CD40 (NP_035741.2) (Met1-Arg193) was expressed with a polyhistidine tag at the C-terminus
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD40 Protein, Mouse, (ECD, His Tag) (50324-M08H) at 2ug/mL (100uL/well) can bind CD40 Ligand Protein, Human, (hFc Tag) (10239-H01H). The EC50 of CD40 L-hFc is 70-180ng/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping: They are shipped out with blue ice pack
Shipping: They are shipped out with blue ice pack
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized CD40 Protein, Mouse, (ECD, His Tag)(50324-M08H) at 2 ug/mL (100 uL/well) can bind CD40 Ligand Protein, Human, (hFc Tag)(10239-H01H), the EC50 of CD40 L-hFc is 70-180 ng/mL.)
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized CD40 Protein, Mouse, (ECD, His Tag)(50324-M08H) at 2 ug/mL (100 uL/well) can bind CD40 Ligand Protein, Human, (hFc Tag)(10239-H01H), the EC50 of CD40 L-hFc is 70-180 ng/mL.)
Application Data
Application Data
Related Product Information for CD40 recombinant protein
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Product Categories/Family for CD40 recombinant protein
References
van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,878 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 1
NCBI Official Synonym Full Names
CD40 antigen
NCBI Official Symbol
Cd40
NCBI Official Synonym Symbols
IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Gene Name
Cd40
UniProt Synonym Gene Names
Tnfrsf5
UniProt Entry Name
TNR5_MOUSE
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Product Notes
The CD40 cd40 (Catalog #AAA257469) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 185aa. The amino acid sequence is listed below: Met1-Arg19 3. It is sometimes possible for the material contained within the vial of "CD40, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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