CD70/CD27 Ligand Recombinant Protein | CD70/CD27 recombinant protein

CD70/CD27 Ligand Protein, Mouse, Recombinant (ECD, His Tag), HPLC-verified

Cat. Num. AAA259263

• Gene Names: Cd70; Cd27l; CD27LG; Tnfsf7
• Purity: >= 95% as determined by SDS-PAGE.; = 90% as determined by SEC-HPLC.
• Synonyms: CD70/CD27 Ligand; N/A; CD70/CD27 Ligand Protein, Mouse, Recombinant (ECD, His Tag), HPLC-verified; CD70 molecule; Cd27l Protein, Mouse; CD27LG Protein, Mouse; Tnfsf7 Protein, Mouse; CD70/CD27 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >= 95% as determined by SDS-PAGE.; = 90% as determined by SEC-HPLC.
• Form/Format: Lyophilized from sterile PBS, pH 7.4.; Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

• Species: Mouse
• Predicted N Terminal: His
• Endotoxin: <1.0 EU per ug protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the Mouse CD70 (NP_035747.1)(Gln47-Pro195) was expressed, with a polyhistidine tag at the N-terminus.
• Activity: Immobilized Recombinant Mouse CD70 Protein (ECD, His Tag)(Cat:51129-M07H) at 2 ug/mL (100 uL/well) can bind Recombinant Mouse CD27/TNFRSF7 Protein (His & Fc Tag)(Cat:50110-M03H), the EC50 is 4-12 ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Application Data

(Immobilized Recombinant Mouse CD70 Protein (ECD, His Tag) (Cat:AAA259263) at 2 ug/mL (100 uL/well) can bind Recombinant Mouse CD27 / TNFRSF7 Protein (His & Fc Tag) (Cat:50110-M03H), the EC50 is 4-12 ng/mL.)

Application Data

Application Data

Related Product Information for CD70/CD27 recombinant protein

CD70, a member of the tumor necrosis factor superfamily, is restricted to activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor, CD27, is important in priming, effector functions, differentiation and memory formation of T-cells as well as plasma and memory B-cell generation. Tight control of CD70 expression is required to prevent lethal immunodeficiency. By selective transcription, CD70 is largely confined to activated lymphocytes and dendritic cells (DC). As a type II transmembrane receptor, CD70 is normally expressed on a subset of B, T and NK cells, where it plays a costimulatory role in immune cell activation. Immunohistochemical analysis of CD70 expression in multiple carcinoma types. The restricted expression pattern of CD70 in normal tissues and its widespread expression in various malignancies makes it an attractive target for antibody-based therapeutics. Investigations to exploit CD70 as a cancer target have lead to the identification of potential antibody-based clinical candidates.

References

Adam PJ, et al. (2006) CD70 (TNFSF7) is expressed at high prevalence in renal cell carcinomas and is rapidly internalised on antibody binding. Br J Cancer. 95(3): 298-306.
Keller AM, et al. (2007) Costimulatory ligand CD70 is delivered to the immunological synapse by shared intracellular trafficking with MHC class II molecules. Proc Natl Acad Sci U S A. 104(14): 5989-94.
Grewal IS. (2008) CD70 as a therapeutic target in human malignancies. Expert Opin Ther Targets. 12(3): 341-51.Boursalian TE, et al. (2009) Targeting CD70 for human therapeutic use. Adv Exp Med Biol. 647: 108-19.

NCBI and Uniprot Product Information

• NCBI GI #: 6755839
• NCBI GeneID: 21948
• NCBI Accession #: NP_035747.1
• NCBI GenBank Nucleotide #: NM_011617.2
• UniProt Accession #: O55237
• Molecular Weight: 21,919 Da
• NCBI Official Full Name: CD70 antigen
• NCBI Official Synonym Full Names: CD70 antigen
• NCBI Official Symbol: Cd70
• NCBI Official Synonym Symbols: Cd27l; CD27LG; Tnfsf7
• NCBI Protein Information: CD70 antigen; CD27 ligand; CD27-L; Ki-24 antigen; tumor necrosis factor (ligand) superfamily, member 7; tumor necrosis factor ligand superfamily member 7
• UniProt Protein Name: CD70 antigen
• UniProt Gene Name: Cd70
• UniProt Synonym Gene Names: Cd27l; Cd27lg; Tnfsf7; CD27-L
• UniProt Entry Name: CD70_MOUSE