SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CDK7/CyclinH1 Recombinant Protein | CDK7 recombinant protein
CDK7/CyclinH1, Active
Applications
Western Blot
Synonyms
CDK7/CyclinH1; N/A; CDK7/CyclinH1, Active; CDK7: CAK1; STK1; CDKN7; p39MO15 Cyclin H1: CCNH; CAK; p34; p37; CDK7 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Applicable Applications for CDK7 recombinant protein
WB (Western Blot), Kinase Assay
Tag
HIS
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for CDK7 recombinant protein
Recombinant full-length human CDK7 and Cyclin H1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tags. The gene accession number for CDK7 and Cyclin H1 are NM_001799 and NM_001239, respectively.
CDK7 gene is a member of the cyclin-dependent protein kinase family that is important regulators of cell cycle progression (1). CDK7 forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). CDK7 is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. CDK7 is thought to serve as a direct link between the regulation of transcription and the cell cycle (2).
CDK7 gene is a member of the cyclin-dependent protein kinase family that is important regulators of cell cycle progression (1). CDK7 forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). CDK7 is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. CDK7 is thought to serve as a direct link between the regulation of transcription and the cell cycle (2).
Product Categories/Family for CDK7 recombinant protein
References
1. Fisher, R. P.: A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase. Cell 78: 713-724, 1994.
2. Larochelle, S.: Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells. Molec. Cell 25: 839-850, 2007.
2. Larochelle, S.: Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells. Molec. Cell 25: 839-850, 2007.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
CDK7 ~41~43kDa
Cyclin H1 ~39kDa
Cyclin H1 ~39kDa
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Product Notes
The CDK7 (Catalog #AAA73356) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CDK7/CyclinH1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the CDK7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK7/CyclinH1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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