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product-image-AAA78574_AD13.png Application Data (Fig-2: Induction of NF-kB activity by TNF-alpha in NF-kB Leeporter GFP reporterHEK293 cells was analyzed by flow cytometry. TNF-alpha was treated at 0.1, 1, 5, 10 and 100 ng/ml as noted. Parental HEK293 cells (Black line, empty); NF-kB/GFP Reporter HEK293 cells (Green line, grey-filled).)

NF-kB Leeporter GFP Reporter-HEK293 Cell Line | NF-kB cell line

NF-kB Leeporter GFP Reporter-HEK293 Cell Line

Applications
Functional Assay
Synonyms
NF-kB Leeporter GFP Reporter-HEK293; N/A; NF-kB Leeporter GFP Reporter-HEK293 Cell Line; NF-kB cell line
Ordering
Form/Format
Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO
Applicable Applications for NF-kB cell line
FA (Functional Assay)
Preparation and Storage
Immediately upon receipt, store in liquid nitrogen.
Ship: Dry ice

Application Data

(Fig-2: Induction of NF-kB activity by TNF-alpha in NF-kB Leeporter GFP reporterHEK293 cells was analyzed by flow cytometry. TNF-alpha was treated at 0.1, 1, 5, 10 and 100 ng/ml as noted. Parental HEK293 cells (Black line, empty); NF-kB/GFP Reporter HEK293 cells (Green line, grey-filled).)

product-image-AAA78574_AD13.png Application Data (Fig-2: Induction of NF-kB activity by TNF-alpha in NF-kB Leeporter GFP reporterHEK293 cells was analyzed by flow cytometry. TNF-alpha was treated at 0.1, 1, 5, 10 and 100 ng/ml as noted. Parental HEK293 cells (Black line, empty); NF-kB/GFP Reporter HEK293 cells (Green line, grey-filled).)

Application Data

(Fig-1: Induction of NF-kB activity by TNF-alpha in NF-kB Leeporter GFP LeeporterHEK293 cells was analyzed by fluorescence microscopy. TNF-alpha was treated at 0.1, 1, 5, 10 and 100 ng/ml as noted.)

product-image-AAA78574_AD15.png Application Data (Fig-1: Induction of NF-kB activity by TNF-alpha in NF-kB Leeporter GFP LeeporterHEK293 cells was analyzed by fluorescence microscopy. TNF-alpha was treated at 0.1, 1, 5, 10 and 100 ng/ml as noted.)
Related Product Information for NF-kB cell line
The NF-kB Leeporter GFP Reporter cell line is a stably transfected HEK293 cell line, which expresses enhanced green fluorescent protein (eGFP) reporter gene under the transcriptional control of the NF-kB response element. NF-kB is a key transcription factor that is involved in immune and inflammatory responses, developmental processes, cellular growth and apoptosis. The NF-kB induction by TNF-alpha is shown in Figures 1 and 2.
Product Categories/Family for NF-kB cell line

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Product Notes

The NF-kB (Catalog #AAA78574) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NF-kB Leeporter GFP Reporter-HEK293 can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay). Researchers should empirically determine the suitability of the NF-kB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-kB Leeporter GFP Reporter-HEK293, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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