Standard Curve (Sample)
(Fig.1. Mouse Nuclear factor-KB p65 (NF-KB p65) Standard Curve.)
Mouse Nuclear Factor-KB p65 (NF-KB p65) ELISA Kit | NF-KB elisa kit
Mouse Nuclear Factor-KB p65 (NF-KB p65) ELISA Kit
Gene Names
Rela; p65; p65 NFkB; p65 NF-kappa B
Reactivity
Mouse
Synonyms
Nuclear Factor-KB p65 (NF-KB p65); N/A; Mouse Nuclear Factor-KB p65 (NF-KB p65) ELISA Kit; RELA; MGC131774; NFKB3; p65; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene; NF-KB elisa kit
Reactivity
Mouse
Specificity
Mouse Nuclear factor-KB p65 (NF-KB p65) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RELA. No significant cross-reactivity or interference between Mouse RELA and analogues was observed.
Sequence Length
549
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Nuclear factor-KB p65 (NF-KB p65) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Nuclear factor-KB p65 (NF-KB p65) Standard Curve.)
Related Product Information for NF-KB elisa kit
Description: This Mouse Nuclear factor-KB p65 (NF-KB p65) ELISA Kit employs a two-site sandwich ELISA to quantitate RELA in samples. An antibody specific for RELA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RELA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA(p65). Four transcript variants encoding different isoforms have been found for RELA(p65).
Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA(p65). Four transcript variants encoding different isoforms have been found for RELA(p65).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transcription factor p65 isoform 1
NCBI Official Synonym Full Names
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
NCBI Official Symbol
Rela
NCBI Official Synonym Symbols
p65; p65 NFkB; p65 NF-kappa B
NCBI Protein Information
transcription factor p65
UniProt Protein Name
Transcription factor p65
UniProt Gene Name
Rela
UniProt Synonym Gene Names
Nfkb3
UniProt Entry Name
TF65_MOUSE
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Product Notes
The Mouse NF-KB rela (Catalog #AAA332392) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332392 ELISA Kit recognizes Mouse NF-KB. It is sometimes possible for the material contained within the vial of "Nuclear Factor-KB p65 (NF-KB p65), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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