Standard Curve (Sample)
(Fig.1. Rat Nuclear factor-kappa B (NF-kB) Standard Curve.)
Rat Nuclear Factor-kappa B (NF-kB) ELISA Kit | NF-Kb elisa kit
Rat Nuclear Factor-kappa B (NF-kB) ELISA Kit
Gene Names
Nfkb1; EBP-1; NF-kB
Reactivity
Rat
Synonyms
Nuclear Factor-kappa B (NF-kB); N/A; Rat Nuclear Factor-kappa B (NF-kB) ELISA Kit; NFKB1; DKFZp686C01211; EBP-1; KBF1; MGC54151; NF-kappa-B; NF-kappaB; NFKB-p105; NFKB-p50; p105; p50; DNA binding factor KBF1; NF-kappabeta; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA bi; NF-Kb elisa kit
Reactivity
Rat
Specificity
Rat Nuclear factor-kappa B (NF-kB) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NFKB1. No significant cross-reactivity or interference between Rat NFKB1 and analogues was observed.
Sequence Length
522
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Rat Nuclear factor-kappa B (NF-kB) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Rat Nuclear factor-kappa B (NF-kB) Standard Curve.)
Related Product Information for NF-Kb elisa kit
Description: This Rat Nuclear factor-kappa B (NF-kB) ELISA Kit employs a two-site sandwich ELISA to quantitate NFKB1 in samples. An antibody specific for NFKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NFKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFKB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Nuclear factor NF-kappa-B p105 subunit isa 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappaB (NF-kappaB) protein complex. NF-kappaB is a transcription factor that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NF-kappaB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions; over 200 known genes are targets of NF-kappaB in various cell types, under specific conditions.
Background: Nuclear factor NF-kappa-B p105 subunit isa 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappaB (NF-kappaB) protein complex. NF-kappaB is a transcription factor that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NF-kappaB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions; over 200 known genes are targets of NF-kappaB in various cell types, under specific conditions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Nuclear factor NF-kappa-B p105 subunit
NCBI Official Synonym Full Names
nuclear factor kappa B subunit 1
NCBI Official Symbol
Nfkb1
NCBI Official Synonym Symbols
EBP-1; NF-kB
NCBI Protein Information
nuclear factor NF-kappa-B p105 subunit
UniProt Protein Name
Nuclear factor NF-kappa-B p105 subunit
UniProt Gene Name
Nfkb1
Similar Products
Product Notes
The Rat NF-Kb nfkb1 (Catalog #AAA330219) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330219 ELISA Kit recognizes Rat NF-Kb. It is sometimes possible for the material contained within the vial of "Nuclear Factor-kappa B (NF-kB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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