Application Data
(Figure 1. NIH3T3/GFP Cell Line. Left: GFP Fluorescence; Right: Phase Contrast)
NIH3T3/GFP Cell Line
NIH3T3/GFP Cell Line
Synonyms
NIH3T3/GFP; N/A; NIH3T3/GFP Cell Line; NIH3T3/GFP cell line
Concentration
1 mL, 1 x 106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO (varies by lot)
Quality Control
This cryovial contains at least 1.0 × 106 NIH3T3/GFP cells as determined by morphology, trypan-blue dye exclusion, and viable cell count. The NIH3T3/GFP cells are tested free of microbial contamination.
Medium
1. Culture Medium: D-MEM (high glucose), 10% fetal bovine serum (FBS), 0.1 mM MEM Non-Essential Amino Acids (NEAA), 2 mM L-glutamine, 1% Pen-Strep, (optional) 10 ug/mL Blasticidin.
2. Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO.
2. Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO.
Methods
Establishing NIH3T3/GFP Cultures from Frozen Cells
1. Place 10 mL of complete DMEM growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1 to 2 minutes by gentle agitation in a 37°C water bath. Decontaminate the cryovial by wiping the surface of the vial with 70% (v/v) ethanol.
2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.
3. Collect the cells by centrifugation at 1000 rpm for 5 minutes at room temperature. Remove the growth medium by aspiration.
4. Resuspend the cells in the conical tube in 15 mL of fresh growth medium by gently pipetting up and down.
5. Transfer the 15 mL of cell suspension to a T-75 tissue culture flask. Place the cells in a 37°C incubator at 5% CO2.
6. Monitor cell density daily. Cells should be passaged when the culture reaches 95% confluence.
1. Place 10 mL of complete DMEM growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1 to 2 minutes by gentle agitation in a 37°C water bath. Decontaminate the cryovial by wiping the surface of the vial with 70% (v/v) ethanol.
2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.
3. Collect the cells by centrifugation at 1000 rpm for 5 minutes at room temperature. Remove the growth medium by aspiration.
4. Resuspend the cells in the conical tube in 15 mL of fresh growth medium by gently pipetting up and down.
5. Transfer the 15 mL of cell suspension to a T-75 tissue culture flask. Place the cells in a 37°C incubator at 5% CO2.
6. Monitor cell density daily. Cells should be passaged when the culture reaches 95% confluence.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Liquid nitrogen
Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80°C until first culture. Store subsequent cultured cells long term in liquid nitrogen.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80°C until first culture. Store subsequent cultured cells long term in liquid nitrogen.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Application Data
(Figure 1. NIH3T3/GFP Cell Line. Left: GFP Fluorescence; Right: Phase Contrast)
Application Data
(Figure 1. NIH3T3/GFP Cell Line. Left: GFP Fluorescence; Right: Phase Contrast)
Related Product Information for NIH3T3/GFP cell line
Background: NIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. The “3T3” designation refers to the abbreviation of "3-day transfer, inoculum 3 x 105 cells." The NIH3T3 cell line is one of the most commonly used fibroblast cell lines. Our NIH3T3/GFP cell line stably expresses GFP and blasticidin-resistant genes. Both GFP and blasticidin-resistant genes are introduced into parental NIH3T3 cells using lentivirus.
Product Categories/Family for NIH3T3/GFP cell line
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Product Notes
The NIH3T3/GFP (Catalog #AAA11425) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NIH3T3/GFP, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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