Application Data
(Image: Lane labelled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated; In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H. Protocol on datasheet.)
Neurofilament NF-H Antibody | anti-NEFH antibody
Neurofilament NF-H, phosphylated, Affinity Purified Antibody, Rabbit
Gene Names
NEFH; NFH; CMT2CC
Reactivity
Chicken, Human, Mouse, Primate, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Synonyms
Neurofilament NF-H; N/A; Neurofilament NF-H, phosphylated, Affinity Purified Antibody, Rabbit; anti-NEFH antibody
Reactivity
Chicken, Human, Mouse, Primate, Rat
Form/Format
Whole serum - Liquid
Sequence Length
1020
Applicable Applications for anti-NEFH antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Type Info
Rabbit IgG
Immunogen
Bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. The form of NF-H isolated is the heavily phosphorylated axonal variant, and the antibody generated recognizes predominantly axonal neurofilaments.
Application Data
(Image: Lane labelled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated; In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H. Protocol on datasheet.)
Application Data
(Image: Lane labelled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated; In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H. Protocol on datasheet.)
Application Data
(Image: Section of rat spinal cord stained with NF-H, phosphylated (green) and monoclonal antibody to UCHL1 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells. Ptotocol on datasheet)
Application Data
(Image: Section of rat spinal cord stained with NF-H, phosphylated (green) and monoclonal antibody to UCHL1 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells. Ptotocol on datasheet)
Related Product Information for anti-NEFH antibody
Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
Product Categories/Family for anti-NEFH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105,639 Da
NCBI Official Full Name
neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament, heavy polypeptide
NCBI Official Symbol
NEFH
NCBI Official Synonym Symbols
NFH; CMT2CC
NCBI Protein Information
neurofilament heavy polypeptide
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Gene Name
NEFH
UniProt Synonym Gene Names
KIAA0845; NFH; NF-H
UniProt Entry Name
NFH_HUMAN
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Product Notes
The NEFH nefh (Catalog #AAA76479) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament NF-H, phosphylated, Affinity Purified Antibody, Rabbit reacts with Chicken, Human, Mouse, Primate, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament NF-H can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the NEFH nefh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament NF-H, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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