Application Data
Mouse Neurofilament NF-H ms Monoclonal Antibody | anti-NEFH antibody
Anti-Neurofilament H (NF-H)
Gene Names
NEFH; NFH
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Protein G Purified tissue culture supernatant.
Synonyms
Neurofilament NF-H ms, Antibody; Anti-Neurofilament H (NF-H); anti-NEFH antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NAP4
Specificity
Specific for the ~200kDa Neurofilament H protein in Western blots of a wide variety of mammals and chicken.
Purity/Purification
Protein G Purified tissue culture supernatant.
Form/Format
100 ul mouse ascites fluid; PBS + 10 mM NaN3
Sequence Length
1020
Applicable Applications for anti-NEFH antibody
ELISA, IF (Immunofluorescence), WB (Western Blot)
Immunogen
Purified bovine neurofilament H
Species Tested
All Mammalian to date, Chicken
Preparation and Storage
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20°C or -80°C, while keeping a working aliquot stored at 4°C for short term. Avoid freeze/thaw cycles.
Application Data
Application Data
Related Product Information for anti-NEFH antibody
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3).
References
• Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
• Mendonca DM, Chimelli L, Martinez AM. (2005) Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis. Braz J Med Biol Res. 38(6):925-933
• Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.
• Mendonca DM, Chimelli L, Martinez AM. (2005) Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis. Braz J Med Biol Res. 38(6):925-933
• Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
200 kDa
NCBI Official Full Name
neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament, heavy polypeptide
NCBI Official Symbol
NEFH
NCBI Official Synonym Symbols
NFH
NCBI Protein Information
neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Gene Name
NEFH
UniProt Synonym Gene Names
KIAA0845; NFH; NF-H
UniProt Entry Name
NFH_HUMAN
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Product Notes
The NEFH nefh (Catalog #AAA72746) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Neurofilament NF-H ms can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the NEFH nefh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament NF-H ms, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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