CLL-1/CLEC12A recombinant protein

CLL-1/CLEC12A Protein, Mouse, Recombinant (His Tag), HPLC-verified

Cat. Num. AAA259252

• Gene Names: Clec12a; Micl; CLL-1; KLRL1; D230024O04
• Purity: >= 95% as determined by SDS-PAGE.; = 95% as determined by SEC-HPLC.
• Synonyms: CLL-1/CLEC12A; N/A; CLL-1/CLEC12A Protein, Mouse, Recombinant (His Tag), HPLC-verified; C-type lectin domain family 12, member A; CLL-1 Protein, Mouse; D230024O04 Protein, Mouse; KLRL1 Protein, Mouse; Micl Protein, Mouse; CLL-1/CLEC12A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >= 95% as determined by SDS-PAGE.; = 95% as determined by SEC-HPLC.
• Form/Format: Lyophilized from sterile PBS, pH 7.4.; Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

• Species: Mouse
• Predicted N Terminal: His
• Endotoxin: <1.0 EU per ug protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the Mouse CLEC12A (NP_808354.1)(Tyr65-Arg267) was expressed, with a polyhistidine tag at the N-terminus.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Application Data

Application Data

Related Product Information for CLL-1/CLEC12A recombinant protein

CLEC12A is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. CLEC12A is a negative regulator of granulocyte and monocyte function. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. C-type lectins are the most diverse and prevalent lectin family in immunity. Using a novel CLEC12A -specific monoclonal antibody, experiments had shown that human CLEC12A was expressed primarily on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells. Although CLEC12A was highly N-glycosylated in primary cells, the level of glycosylation was found to vary between cell types. CLEC12A surface expression was down-regulated during inflammatory/activation conditions in vitro, as well as during an in vivo model of acute inflammation. This suggests that CLEC12A may be involved in the control of myeloid cell activation during inflammation.

References

Lahoud MH, et al. (2009) The C-type lectin Clec12A present on mouse and human dendritic cells can serve as a target for antigen delivery and enhancement of antibody responses. J Immunol. 182(12): 7587-94.
Pyz E, et al. (2008) Characterisation of murine MICL (CLEC12A) and evidence for an endogenous ligand. Eur J Immunol. 38(4): 1157-63.Marshall AS, et al. (2006) Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation. Eur J Immunol. 36(8): 2159-69.

NCBI and Uniprot Product Information

• NCBI GI #: 29244122
• NCBI GeneID: 232413
• NCBI Accession #: NP_808354.1
• NCBI GenBank Nucleotide #: NM_177686.4
• UniProt Accession #: Q504P2; Q8BN96
• Molecular Weight: 30,757 Da
• NCBI Official Full Name: C-type lectin domain family 12 member A
• NCBI Official Synonym Full Names: C-type lectin domain family 12, member a
• NCBI Official Symbol: Clec12a
• NCBI Official Synonym Symbols: Micl; CLL-1; KLRL1; D230024O04
• NCBI Protein Information: C-type lectin domain family 12 member A; C-type lectin-like molecule 1; myeloid inhibitory C-type lectin-like receptor
• UniProt Protein Name: C-type lectin domain family 12 member A
• UniProt Gene Name: Clec12a
• UniProt Synonym Gene Names: Micl; CLL-1; MICL
• UniProt Entry Name: CL12A_MOUSE