SDS-PAGE
Pesticidal crystal protein cry3Aa Recombinant Protein | cry3Aa recombinant protein
Recombinant Pesticidal crystal protein cry3Aa
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Pesticidal crystal protein cry3Aa; N/A; Recombinant Pesticidal crystal protein cry3Aa; 73 kDa crystal protein; Crystaline entomocidal protoxinInsecticidal delta-endotoxin CryIIIA(a); cry3Aa recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
58-644aa, Full Length of Mature Protein
Sequence
DSSTTKDVIQKGISVVGDLLGVVGFPFGGALVSFYTNFLNTIWPSEDPWKAFMEQVEALMDQKIADYAKNKALAELQGLQNNVEDYVSALSSWQKNPVSSRNPHSQGRIRELFSQAESHFRNSMPSFAISGYEVLFLTTYAQAANTHLFLLKDAQIYGEEWGYEKEDIAEFYKRQLKLTQEYTDHCVKWYNVGLDKLRGSSYESWVNFNRYRREMTLTVLDLIALFPLYDVRLYPKEVKTELTRDVLTDPIVGVNNLRGYGTTFSNIENYIRKPHLFDYLHRIQFHTRFQPGYYGNDSFNYWSGNYVSTRPSIGSNDIITSPFYGNKSSEPVQNLEFNGEKVYRAVANTNLAVWPSAVYSGVTKVEFSQYNDQTDEASTQTYDSKRNVGAVSWDSIDQLPPETTDEPLEKGYSHQLNYVMCFLMQGSRGTIPVLTWTHKSVDFFNMIDSKKITQLPLVKAYKLQSGASVVAGPRFTGGDIIQCTENGSAATIYVTPDVSYSQKYRARIHYASTSQITFTLSLDGAPFNQYYFDKTINKGDTLTYNSFNLASFSTPFELSGNNLQIGVTGLSAGDKVYIDKIEFIPVN
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for cry3Aa recombinant protein
Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera.
References
Nucleotide sequence of a gene encoding an insecticidal protein of Bacillus thuringiensis var. tenebrionis toxic against Coleoptera.Hoefte H., Seurinck J., Houtven A.V., Vaeck M.Nucleic Acids Res. 15:7183-7183(1987) Molecular cloning and characterization of the insecticidal crystal protein gene of Bacillus thuringiensis var. tenebrionis.Sekar V., Thompson D.V., Maroney M.J., Bookland R.G., Adang M.J.Proc. Natl. Acad. Sci. U.S.A. 84:7036-7040(1987) Characterization of the coleopteran-specific protein gene of Bacillus thuringiensis var. tenebrionis.McPherson S.A., Perlak F.J., Fuchs R.L., Marrone P.G., Lavrik P.B., Fischhoff D.A.Biotechnology (N.Y.)6:61-66(1988) Elucidation of the mechanism of CryIIIA overproduction in a mutagenized strain of Bacillus thuringiensis var. tenebrionis.Adams L.F., Mathewes S., O'Hara P., Petersen A., Gurtler H.Mol. Microbiol. 14:381-389(1994) Crystal structure of insecticidal delta-endotoxin from Bacillus thuringiensis at 2.5-A resolution.Li J., Carroll J., Ellar D.J.Nature 353:815-821(1991)
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
68.5 kDa
NCBI Official Full Name
Pesticidal crystal protein Cry3Aa
UniProt Protein Name
Pesticidal crystal protein Cry3Aa
UniProt Gene Name
cry3Aa
UniProt Synonym Gene Names
bt13; cry3A; cryC; cryIIIa; cryIIIA(a)
UniProt Entry Name
CR3AA_BACTT
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Product Notes
The cry3Aa cry3aa (Catalog #AAA114771) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 58-644aa, Full Length of Mature Protein. The amino acid sequence is listed below: DSSTTKDVIQ KGISVVGDLL GVVGFPFGGA LVSFYTNFLN TIWPSEDPWK AFMEQVEALM DQKIADYAKN KALAELQGLQ NNVEDYVSAL SSWQKNPVSS RNPHSQGRIR ELFSQAESHF RNSMPSFAIS GYEVLFLTTY AQAANTHLFL LKDAQIYGEE WGYEKEDIAE FYKRQLKLTQ EYTDHCVKWY NVGLDKLRGS SYESWVNFNR YRREMTLTVL DLIALFPLYD VRLYPKEVKT ELTRDVLTDP IVGVNNLRGY GTTFSNIENY IRKPHLFDYL HRIQFHTRFQ PGYYGNDSFN YWSGNYVSTR PSIGSNDIIT SPFYGNKSSE PVQNLEFNGE KVYRAVANTN LAVWPSAVYS GVTKVEFSQY NDQTDEASTQ TYDSKRNVGA VSWDSIDQLP PETTDEPLEK GYSHQLNYVM CFLMQGSRGT IPVLTWTHKS VDFFNMIDSK KITQLPLVKA YKLQSGASVV AGPRFTGGDI IQCTENGSAA TIYVTPDVSY SQKYRARIHY ASTSQITFTL SLDGAPFNQY YFDKTINKGD TLTYNSFNLA SFSTPFELSG NNLQIGVTGL SAGDKVYIDK IEFIPVN. It is sometimes possible for the material contained within the vial of "Pesticidal crystal protein cry3Aa, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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