Ames Medium w/L-Glutamine Culture Media
Ames Medium w/L-Glutamine (Powder)
Purity
Cell Culture Grade
Synonyms
Ames Medium w/L-Glutamine; N/A; Ames Medium w/L-Glutamine (Powder); Ames Medium w/L-Glutamine culture media
Purity/Purification
Cell Culture Grade
Endotoxin Level
<1EU/ml
Appearance
White, homogenous, free flowing powder
Solubility
Colorless, clear, complete
pH
4.5
Directions per Liter
Gas ~900ml of water (15-20°C) with 100% CO2. While gently stirring the water, add 1.9g sodium bicarbonate. Add 8.8g of the powder medium and stir until dissolved. Adjust the pH of the medium to 0.2-0.3 pH unit below the desired level using 1N NaOH and 1N HCl. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components Shown As g/liter
Inorganic Salts
Calcium Chloride - 0.1275
Magnesium Sulfate - 0.1488
Potassium Chloride - 0.231
Potassium Phosphate Mono. - 0.068
Sodium Chloride - 7.01
Amino Acids
L-Alanine - 0.0024
L-Arginine•HCl - 0.00421
L-Aspartic Acid - 0.00012
L-Asparagine - 0.00084
L-Cystine•2HCl - 0.000065
L-Glutamic Acid, Sodium - 0.001183
L-Glutamine - 0.073
Glycine - 0.00045
L-Histidine•HCl•H2O - 0.002513
L-Isoleucine - 0.00058
L-Leucine - 0.00144
L-Lysine•HCl - 0.003648
L-Methionine - 0.00039
L-Phenylalanine - 0.00132
L-Proline - 0.00007
L-Serine - 0.00252
Taurine - 0.00075
L-Threonine - 0.00333
L-Tryptophan - 0.00049
L-Tyrosine•2Na•2H2O - 0.00211
L-Valine - 0.00176
Vitamins
Ascorbic Acid•Na - 0.01796
D-Biotin - 0.0001
Choline Chloride - 0.0007
Folic Acid - 0.0001
myo-Inositol - 0.0272
Niacinamide - 0.0001
D-Pantothenic Acid•Ca - 0.0001
Pyridoxal•HCl - 0.0001
Riboflavin - 0.00001
Thiamine•HCl - 0.0001
Other
Cytidine - 0.00073
Thymidine - 0.00024
D-Glucose - 1.081
Hypoxanthine - 0.00082
Pyruvic Acid, Sodium - 0.01333
Uridine - 0.00073
Total: 8.8 g/liter
Calcium Chloride - 0.1275
Magnesium Sulfate - 0.1488
Potassium Chloride - 0.231
Potassium Phosphate Mono. - 0.068
Sodium Chloride - 7.01
Amino Acids
L-Alanine - 0.0024
L-Arginine•HCl - 0.00421
L-Aspartic Acid - 0.00012
L-Asparagine - 0.00084
L-Cystine•2HCl - 0.000065
L-Glutamic Acid, Sodium - 0.001183
L-Glutamine - 0.073
Glycine - 0.00045
L-Histidine•HCl•H2O - 0.002513
L-Isoleucine - 0.00058
L-Leucine - 0.00144
L-Lysine•HCl - 0.003648
L-Methionine - 0.00039
L-Phenylalanine - 0.00132
L-Proline - 0.00007
L-Serine - 0.00252
Taurine - 0.00075
L-Threonine - 0.00333
L-Tryptophan - 0.00049
L-Tyrosine•2Na•2H2O - 0.00211
L-Valine - 0.00176
Vitamins
Ascorbic Acid•Na - 0.01796
D-Biotin - 0.0001
Choline Chloride - 0.0007
Folic Acid - 0.0001
myo-Inositol - 0.0272
Niacinamide - 0.0001
D-Pantothenic Acid•Ca - 0.0001
Pyridoxal•HCl - 0.0001
Riboflavin - 0.00001
Thiamine•HCl - 0.0001
Other
Cytidine - 0.00073
Thymidine - 0.00024
D-Glucose - 1.081
Hypoxanthine - 0.00082
Pyruvic Acid, Sodium - 0.01333
Uridine - 0.00073
Total: 8.8 g/liter
Preparation and Storage
Store powdered media at 4°C. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Ames Medium w/L-Glutamine culture media
The retina is an important in vitro model for the central nervous system. It is more readily accessible than most nervous tissue and is strong enough to remain intact during manipulations. The retina of the rabbit commonly contains no penetrating blood vessels. It is nurtured by diffusion from capillary networks on either side. For this reason, the tissue will survive and functions without circulating blood, as long as it is bathed in a medium that closely resembles in vivo conditions.
Ames' medium was formulated to support retinal tissue in relatively short-term culture. Rabbit retina has been incubated in Ames' medium for over 2 days with its metabolism and electrical responses to light stimuli well maintained. This mixture is a medium of choice for maintaining central nervous system tissue in vitro.
Ames' medium was formulated to support retinal tissue in relatively short-term culture. Rabbit retina has been incubated in Ames' medium for over 2 days with its metabolism and electrical responses to light stimuli well maintained. This mixture is a medium of choice for maintaining central nervous system tissue in vitro.
Product Categories/Family for Ames Medium w/L-Glutamine culture media
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Product Notes
The Ames Medium w/L-Glutamine (Catalog #AAA14826) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ames Medium w/L-Glutamine, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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