Annexin V Apoptosis Detection Kit

Annexin V PE Apoptosis Detection Kit

Cat. Num. AAA79344

• Applications: Flow Cytometry, Functional Assay
• Purity: The product should be stored undiluted at 4 degree C and should be protected from prolonged exposure to light.
• Synonyms: Annexin V Apoptosis; N/A; Annexin V PE Apoptosis Detection Kit; Annexin V Kit; Annexin V Apoptosis detection kit

• Purity/Purification: The product should be stored undiluted at 4 degree C and should be protected from prolonged exposure to light.
• Form/Format: Aqueous buffered solution containing 1mM EDTA, 10mM HEPES, 0.09% NaN3.
• Conjugation: PE

• Applicable Applications for Annexin V Apoptosis detection kit: FCM/FACS (Flow Cytometry)
• Preparation and Storage: The product should be stored undiluted at 4 degree C and should be protected from prolonged exposure to light.

FCM/FACS (Flow Cytometry)

Related Product Information for Annexin V Apoptosis detection kit

Principle of the Assay: Annexin V is a phospholipid phosphatidylserine (PS) binding protein. In the earlier stages of apoptosis, PS is translocated to the outer sections of the cell plasma membrane and is exposed to the external cellular environment. The mark of the late stages of cellular death is the loss of membrane integrity. Cells can be stained with Annexin V conjugated to a fluorochrome together with 7-AAD, which is excluded from viable cells with intact membranes. This can be used to distinguish cells that are viable (Annexin V and 7-AAD negative), early apoptotic (Annexin V positive and 7-AAD negative), and those that are dead or in late apoptosis (Annexin V and 7-AAD positive). Annexin V binding alone cannot differentiate between necrotic and apoptotic cells. The Annexin V Apoptosis Detection Kit contains: Annexin V PE (100 tests), Annexin V Binding Buffer 1X (50mL), and 7-AAD (200 tests).