Application Data
(Fig.2: Adherent RAW cells were treated with 0.05 ?g/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Celltase and stained with the Annexin VFITC Apoptosis Detection Kit.)
Annexin V-FITC Apoptosis Detection Kit
Annexin V-FITC Apoptosis Detection Kit
Applications
Flow Cytometry, Functional Assay
Synonyms
Annexin V-FITC Apoptosis; N/A; Annexin V-FITC Apoptosis Detection Kit; Annexin V-FITC Apoptosis detection kit
Applicable Applications for Annexin V-FITC Apoptosis detection kit
FCM/FACS (Flow Cytometry)
Preparation and Storage
Store all kit components at 4 degree C.
Application Data
(Fig.2: Adherent RAW cells were treated with 0.05 ?g/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Celltase and stained with the Annexin VFITC Apoptosis Detection Kit.)
Application Data
(Fig.2: Adherent RAW cells were treated with 0.05 ?g/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Celltase and stained with the Annexin VFITC Apoptosis Detection Kit.)
Application Data
(Fig.1: Control Cells were stained or unstained as indicated using the Annexin V-FITC Apoptosis Detection Kit.)
Application Data
(Fig.1: Control Cells were stained or unstained as indicated using the Annexin V-FITC Apoptosis Detection Kit.)
Related Product Information for Annexin V-FITC Apoptosis detection kit
Principle of the Assay: Apoptosis or programmed cell death is an evolutionary conserved, intrinsic program of cell death that occurs in various physiological and pathological situations. It is a key regulator of physiological growth control and regulation of tissue homeostasis. Apoptosis is considered a vital component of various processes including normal cell turnover, proper development and functioning of the immune system, hormone-dependent atrophy, embryonic development and chemicalinduced cell death. The term apoptosis (a-po-toe-sis) was first used in a now-classic paper by Kerr, Wyllie, and Currie in 1972 to describe a morphologically distinct form of cell death, although certain components of the apoptosis concept had been explicitly described many years previously. Apoptosis is characterized by specific morphological changes of the dying cells; namely, loss of membrane asymmetry, cytoskeleton remodeling, plasma membrane blebbing, loss of the mitochondrial membrane potential, caspase activation, chromatin condensation, and DNA fragmentation. During apoptosis, the asymmetric distribution of phospholipids of the plasma membrane gets lost and phosphatidylserine (PS) is translocated to the outer leaflet of the plasma membrane. There, PS acts as one major "eat me" signal that ensures efficient recognition and uptake of apoptotic cells by phagocytes. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis.
Product Categories/Family for Annexin V-FITC Apoptosis detection kit
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Product Notes
The Annexin V-FITC Apoptosis (Catalog #AAA78385) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Annexin V-FITC Apoptosis can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the Annexin V-FITC Apoptosis for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin V-FITC Apoptosis, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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