Application Data
Annexin V-FITC / PI Apoptosis Detection Kit
Annexin V-FITC / PI Apoptosis Detection Kit
Synonyms
Annexin V-FITC / PI Apoptosis Detection Kit; N/A; Annexin V-FITC / PI Apoptosis Detection Kit detection kit
Application Data
Application Data
Application Data
Application Data
Related Product Information for Annexin V-FITC / PI Apoptosis Detection Kit detection kit
Background/Introduction: Annexin A5 (or Annexin V) is a cellular protein in the annexin group. Annexin A5 is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (annexin V binding to PS is Ca2+ dependent). The assay combines annexin V staining of PS membrane events with the staining of cell nucleus with PI or AAD-7 in living cells to distinguish from dead cells. Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy.
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Product Notes
The Annexin V-FITC / PI Apoptosis Detection Kit (Catalog #AAA58858) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Annexin V-FITC / PI Apoptosis Detection Kit, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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