IF (Immunofluorescence)
(Fluorescence images using TdT and Fluorescence-labeled dUTP in Hela cells with the treatment of 10 U/mL Dnase I for 10min. The cells were counterstained with DAPI.)
Terminal Deoxynucleotidyl Transferase (TdT) Recombinant Protein | TdT recombinant protein
Terminal Deoxynucleotidyl Transferase (TdT)
Purity
> 90% as determined by SDS-PAGE
Synonyms
Terminal Deoxynucleotidyl Transferase (TdT); N/A; TdT; DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase; TdT recombinant protein
Host
E. coli
Purity/Purification
> 90% as determined by SDS-PAGE
Form/Format
The recombinant bovine TdT reconstitution solution, the compound solution is a liquid in sterile aqueous solution, 50 mM K2HPO4, 100 mM NaCl, 1 mM DTT, 0.1% Tween20, 1% BSA, 50% Glycerol, pH6.5.
Tag
TdT
Protein Construction
The recombinant bovine TdT consists of 509 amino acids and migrates with an apparent molecular mass of 58.3kDa as estimated in SDS-PAGE under reducing conditions.
Unit Definition
One unit of the enzyme catalyzes the incorporation of 1 nmol of deoxythymidylate into a polynucleotide fraction in 60 min at 37°C.
Usage Notes
The Equilibration Buffer (5×) stock solution is stored at low temperature, resulting in a small amount of component precipitation. Please invert and mix before use. And Equilibration Buffer (5×) contains cacodylate and cobalt chloride, highly toxic chemicals. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately.
Preparation and Storage
Store at -20 degree C.
IF (Immunofluorescence)
(Fluorescence images using TdT and Fluorescence-labeled dUTP in Hela cells with the treatment of 10 U/mL Dnase I for 10min. The cells were counterstained with DAPI.)
IF (Immunofluorescence)
(Fluorescence images using TdT and Fluorescence-labeled dUTP in Hela cells with the treatment of 10 U/mL Dnase I for 10min. The cells were counterstained with DAPI.)
Application Data
(Formulation)
Application Data
(Formulation)
SDS-PAGE
(Fig. SDS-PAGE analysis of TdT.)
SDS-PAGE
(Fig. SDS-PAGE analysis of TdT.)
Related Product Information for TdT recombinant protein
Description: Terminal Deoxynucleotidyl Transferase (TdT), expressed in Null
Background: Terminal deoxynucleotidyl transferase (TdT), also known as terminal transferase, is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphoma cells. Generally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases, it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends.
Background: Terminal deoxynucleotidyl transferase (TdT), also known as terminal transferase, is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphoma cells. Generally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases, it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends.
Product Categories/Family for TdT recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,296 Da
NCBI Official Full Name
DNA nucleotidylexotransferase
NCBI Official Synonym Full Names
DNA nucleotidylexotransferase
NCBI Official Symbol
DNTT
NCBI Protein Information
DNA nucleotidylexotransferase
UniProt Protein Name
DNA nucleotidylexotransferase
UniProt Gene Name
DNTT
UniProt Synonym Gene Names
TDT; TDT; Terminal transferase
Similar Products
Product Notes
The TdT dntt (Catalog #AAA332528) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Terminal Deoxynucleotidyl Transferase (TdT), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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