cIAP1 / HIAP2 active protein

Recombinant Human cIAP1 / HIAP2 Protein (AVI tag)

Cat. Num. AAA173550

• Gene Names: BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
• Purity: > 92 % as determined by reducing SDS-PAGE.
• Synonyms: cIAP1 / HIAP2; N/A; Recombinant Human cIAP1 / HIAP2 Protein (AVI tag); API1; c-IAP1; cIAP1; Hiap-2; HIAP2; MIHB; RNF48; cIAP1 / HIAP2 active protein

• Host: E.Coli
• Purity/Purification: > 92 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile 20mM Tris, 300mM NaCl, pH 8.0, 5%Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence Positions: Glu 144-Leu 356

• Species: Human
• Bio_activity: Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP. The IC50 for this effect is typically 25-750 nM.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. ; It is recommended that sterile water(400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Ships on blue ice. Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for cIAP1 / HIAP2 active protein

The cellular inhibitor of apoptosis protein-1 (cIAP1) is a member of the Inhibitor of Apoptosis family proteinsare (IAP) whose members are characterized by a novel domain of about 70 amino acids termed baculoviral IAP repeats (BIRs). The BIR domains of cIAP1 and cIAP2 bind to caspases, the key effector proteases of apoptosis. The IAP protein family which can enhance cell survival are crucial regulators of programmed cell death. Both cIAP1 and cIAP2 are the E3 ubiquitin protein isopeptide ligases for Smac, taking part in promoting cancer survival through functioning as E3 ubiqitin ligases. Removal of cIAP1 by genetic deletion may result in NF-?B signaling activation that induces TNF? production and in killing sensitive tumor cells through enhanced TNF-R1 death-receptor signaling and caspase 8 activation. The substrate-dependent E3 activity of cIAPs is mediated by their RING domains and is dependent on the specific interactions between cIAPs and Smac. cIAP1 and cIAP2 are also reported to be regulators of NF-kB activation upon TNF α treatment.

NCBI and Uniprot Product Information

• NCBI GI #: 4502141
• NCBI GeneID: 329
• NCBI Accession #: NP_001157.1
• NCBI GenBank Nucleotide #: NM_001166.4
• UniProt Accession #: Q13490; Q16516; Q4TTG0; B4E026
• Molecular Weight: Mol_Mass: 26.5 kDa; AP_Mol_Mass: 26.5 kDa
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 2
• NCBI Official Symbol: BIRC2
• NCBI Official Synonym Symbols: API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
• NCBI Protein Information: baculoviral IAP repeat-containing protein 2
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 2
• UniProt Gene Name: BIRC2
• UniProt Synonym Gene Names: API1; MIHB; RNF48; hIAP-2; hIAP2