SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Hydroxyacid Oxidase-1/HAO-1 Active Protein | HAO-1 active protein
Recombinant Hydroxyacid Oxidase-1/HAO-1
Gene Names
Hao1; GOX; Gox1; Hao-1
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
Hydroxyacid Oxidase-1/HAO-1; N/A; Recombinant Hydroxyacid Oxidase-1/HAO-1; (S)-2-hydroxy-acid oxidase; EC 1.1.3.15; Glycolate oxidase; GOX; GOX1MGC142227; GOXMGC142225; HAO1; HAO-1; HAOX1; hydroxyacid oxidase (glycolate oxidase) 1; hydroxyacid oxidase 1; Hydroxyacid Oxidase1; Hydroxyacid Oxidase-1; HAO-1 active protein
Host
E Coli
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In Phosphate-Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
1-370aa
Sequence
MGSSHHHHHH SSGLVPRGSH MGS MLPRLVC ISDYEQHVRS VLQKSVYDYY RSGANDQETL ADNIQAFSRW KLYPRMLRNV ADIDLSTSVL GQRVSMPICV GATAMQCMAH VDGELATVRA CQTMGTGMML SSWATSSIEE VAEAGPEALR WMQLYIYKDR EISRQIVKRA EKQGYKAIFV TVDTPYLGNR IDDVRNRFKL PPQLRMKNFE TNDLAFSPKG NFGDNSGLAE YVAQAIDPSL SWDDITWLRR LTSLPIVVKG ILRGDDAKEA VKHGVDGILV SNHGARQLDG VPATIDVLPE IVEAVEGKVE VFLDGGVRKG TDVLKALALG AKAVFVGRPI IWGLAFQGEK GVQDVLEILK EEFRLAMALS GCQNVKVIDK TLVRKNPLAV SKI
Sequence Length
370
Applicable Applications for HAO-1 active protein
Enzyme Activity, SDS-PAGE
Species
Mouse
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Biological Activity
Specific activity is > 1,000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C.
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for HAO-1 active protein
Hydroxyacid Oxidase-1/HAO-1, also known as hydroxyacid oxidase 1, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on two carbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant mouse Hydroxyacid Oxidase-1/HAO-1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for HAO-1 active protein
References
Pennati A., et al. (2009) J. Biol. Chem. 284(45):31214-22. Murray MS., et al. (2008) Biochemistry. 47(8):2439-49.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43.4 kDa (393aa), confirmed by MALDI-TOF
NCBI Official Full Name
hydroxyacid oxidase 1
NCBI Official Synonym Full Names
hydroxyacid oxidase 1, liver
NCBI Official Symbol
Hao1
NCBI Official Synonym Symbols
GOX; Gox1; Hao-1
NCBI Protein Information
hydroxyacid oxidase 1
UniProt Protein Name
Hydroxyacid oxidase 1
UniProt Gene Name
Hao1
UniProt Synonym Gene Names
Gox1; Hao-1; HAOX1; GOX
UniProt Entry Name
HAOX1_MOUSE
Similar Products
Product Notes
The HAO-1 hao1 (Catalog #AAA48314) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-370aa. AAA Biotech's Hydroxyacid Oxidase-1/HAO-1 can be used in a range of immunoassay formats including, but not limited to, Enzyme Activity, SDS-PAGE. Researchers should empirically determine the suitability of the HAO-1 hao1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGS MLPRLVC ISDYEQHVRS VLQKSVYDYY RSGANDQETL ADNIQAFSRW KLYPRMLRNV ADIDLSTSVL GQRVSMPICV GATAMQCMAH VDGELATVRA CQTMGTGMML SSWATSSIEE VAEAGPEALR WMQLYIYKDR EISRQIVKRA EKQGYKAIFV TVDTPYLGNR IDDVRNRFKL PPQLRMKNFE TNDLAFSPKG NFGDNSGLAE YVAQAIDPSL SWDDITWLRR LTSLPIVVKG ILRGDDAKEA VKHGVDGILV SNHGARQLDG VPATIDVLPE IVEAVEGKVE VFLDGGVRKG TDVLKALALG AKAVFVGRPI IWGLAFQGEK GVQDVLEILK EEFRLAMALS GCQNVKVIDK TLVRKNPLAV SKI. It is sometimes possible for the material contained within the vial of "Hydroxyacid Oxidase-1/HAO-1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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