Bioactivity
(Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family. PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L). A small portion of PSA "leaks" into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue. PSA has become a well established tumor marker that aids the identification, staging, and follow up of prostate cancer. The activity of recombinant human KLK3 is measured by its ability to cleave a colorimetric peptide substrate Suc-Arg-Pro-Tyr-pNa in the assay buffer 50 mM Tris, 1 M NaCl, pH 8.0. The rhKLK3 is diluted to 200 ug/ml in activation buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5, then activated with a final concentration of 1 ug/ml Thermolysin at 37 degree C for 5min. Adding a final concentration of 10 mM 1,10 Phenanthroline to stop the activation. The activated rhKLK3 is diluted to 50 ug/mL in assay buffer. Loading into a clear well plate 50 uL of 50 ug/mL rhKLK3 and start the reaction by adding 50 uL of 2 mM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhKLK3. Then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant human KLK3 is > 400 pmol/min/ug.)
Prostate Specific Antigen (PSA) Active Protein | PSA active protein
Active Prostate Specific Antigen (PSA)
Gene Names
KLK3; APS; PSA; hK3; KLK2A1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Prostate Specific Antigen (PSA); N/A; Active Prostate Specific Antigen (PSA); Prostate Specific Antigen; KLK3; APS; KLK2A1; HK3; KLKB1; Seminin; Kallikrein-Related Peptidase 3; Semenogelase, g-Seminoprotein; P-30 Antigen; Plasma kallikrein4; PSA active protein
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Ile25~Pro261
Applicable Applications for PSA active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
7.5
Research Area
Tumor immunity, Reproductive science
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family. PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L). A small portion of PSA "leaks" into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue. PSA has become a well established tumor marker that aids the identification, staging, and follow up of prostate cancer. The activity of recombinant human KLK3 is measured by its ability to cleave a colorimetric peptide substrate Suc-Arg-Pro-Tyr-pNa in the assay buffer 50 mM Tris, 1 M NaCl, pH 8.0. The rhKLK3 is diluted to 200 ug/ml in activation buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5, then activated with a final concentration of 1 ug/ml Thermolysin at 37 degree C for 5min. Adding a final concentration of 10 mM 1,10 Phenanthroline to stop the activation. The activated rhKLK3 is diluted to 50 ug/mL in assay buffer. Loading into a clear well plate 50 uL of 50 ug/mL rhKLK3 and start the reaction by adding 50 uL of 2 mM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhKLK3. Then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant human KLK3 is > 400 pmol/min/ug.)
Bioactivity
(Kallikrein 3, commonly known as prostate specific antigen (PSA), is a serine protease of the human tissue Kallikrein gene family. PSA is synthesized in the ductal and acinar epithelium of the prostate gland and secreted into the seminal plasma in high concentrations (0.5 - 2 g/L). A small portion of PSA "leaks" into the systemic circulation, the levels of which increase significantly (30-fold) from prostate cancer tissue than normal prostate tissue. PSA has become a well established tumor marker that aids the identification, staging, and follow up of prostate cancer. The activity of recombinant human KLK3 is measured by its ability to cleave a colorimetric peptide substrate Suc-Arg-Pro-Tyr-pNa in the assay buffer 50 mM Tris, 1 M NaCl, pH 8.0. The rhKLK3 is diluted to 200 ug/ml in activation buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5, then activated with a final concentration of 1 ug/ml Thermolysin at 37 degree C for 5min. Adding a final concentration of 10 mM 1,10 Phenanthroline to stop the activation. The activated rhKLK3 is diluted to 50 ug/mL in assay buffer. Loading into a clear well plate 50 uL of 50 ug/mL rhKLK3 and start the reaction by adding 50 uL of 2 mM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhKLK3. Then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant human KLK3 is > 400 pmol/min/ug.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for PSA active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,741 Da
NCBI Official Full Name
prostate-specific antigen isoform 3 preproprotein
NCBI Official Synonym Full Names
kallikrein-related peptidase 3
NCBI Official Symbol
KLK3
NCBI Official Synonym Symbols
APS; PSA; hK3; KLK2A1
NCBI Protein Information
prostate-specific antigen; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen
UniProt Protein Name
Prostate-specific antigen
UniProt Gene Name
KLK3
UniProt Synonym Gene Names
APS; PSA; Seminin
UniProt Entry Name
KLK3_HUMAN
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Product Notes
The PSA klk3 (Catalog #AAA161711) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Prostate Specific Antigen (PSA) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the PSA klk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ile25~Pro2 61. It is sometimes possible for the material contained within the vial of "Prostate Specific Antigen (PSA), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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