UCHL1 active protein

UCHL1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257988

• Gene Names: UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
• Purity: >92% as determined by SDS-PAGE
• Synonyms: UCHL1; N/A; UCHL1 Protein, Human, Recombinant (His Tag); HEL-117; NDGOA; PARK5; PGP9.5; PGP95; Uch-L1; Human UCHL1/PGP9.5 Protein (His Tag); HEL-117 Protein, Human; NDGOA Protein, Human; PARK5 Protein, Human; PGP9.5 Protein, Human; PGP95 Protein, Human; Uch-L1 Protein, Human; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); UCHL1 active protein

• Host: E Coli
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0
• Concentration: 0.71mg/mL (varies by lot)
• Sequence: Gln2-Ala223

• Source Note: E.coli-derived Human UCHL1 / PGP9.5 Protein (His Tag)(Gln2-Ala223, Accesion# NP_004172.2)
• Activity: Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/ug.
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the native human UCHL1 (NP_004172.2)(Gln 2-Ala 223) was expressed, with a polyhistide tag at the N-terminus.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for UCHL1 active protein

Background: Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

References

Wada H., et al., 1998, Biochem. Biophys. Res. Commun. 251:688-92. Choi J., et al., 2004, J. Biol. Chem. 279:13256-64. Lombardino,A.2005, et al., J.Proc Natl Acad Sci.USA102 (22):8036-41 Okochi-Takada,E. et al., 2006, Int J Cancer. 119 (6):1338-44. Zetterberg, M. et al., 2010, Mol Neurodegener 5 :11.

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172.2
• NCBI GenBank Nucleotide #: NM_004181.4
• UniProt Accession #: P09936; Q4W5K6; Q71UM0
• Molecular Weight: Predicted Molecular Mass: 25.6 kDa; Molecular Mass Note: The recombinant human UCHL1 consisting of 229 amino acids and migrates as an 25.6 kDa band in SDS-PAGE under reducing conditions as predicted.
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN