Epithelial Neutrophil-Activating Protein 78 Active Protein | ENA 78 active protein
Recombinant Human Epithelial Neutrophil-Activating Protein 78 (CXCL5)
Gene Names
CXCL5; SCYB5; ENA-78
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
Epithelial Neutrophil-Activating Protein 78; N/A; Recombinant Human Epithelial Neutrophil-Activating Protein 78 (CXCL5); ENA 78 Human; Epithelial Neutrophil-Activating Protein 78 Human Recombinant (CXCL5); Small inducible cytokine B5; CXCL5; Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; ENA-78(1-78); chemokine (C-X-C motif) ligand 5; SCYB5; ENA 78 active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The CXCL5 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-Arg
Sequence Length
114
Solubility
It is recommended to reconstitute the lyophilized ENA-78 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with 100ng/mL (corresponding to a Specific Activity of 10,000IU/mg) of recombinant human ENA-78.
Preparation and Storage
Lyophilized ENA78 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CXCL5 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for ENA 78 active protein
Description: Epithelial Neutrophil-Activating Protein 78 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton. The CXCL5 is purified by proprietary chromatographic techniques.
Introduction: Chemokine (C-X-C motif) ligand 5 (CXCL5) is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-?. This chemokine stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodelling.
Introduction: Chemokine (C-X-C motif) ligand 5 (CXCL5) is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-?. This chemokine stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodelling.
Product Categories/Family for ENA 78 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,972 Da
NCBI Official Full Name
C-X-C motif chemokine 5
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 5
NCBI Official Symbol
CXCL5
NCBI Official Synonym Symbols
SCYB5; ENA-78
NCBI Protein Information
C-X-C motif chemokine 5; epithelial-derived neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
UniProt Protein Name
C-X-C motif chemokine 5
UniProt Gene Name
CXCL5
UniProt Synonym Gene Names
ENA78; SCYB5
UniProt Entry Name
CXCL5_HUMAN
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Product Notes
The ENA 78 cxcl5 (Catalog #AAA38090) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-A rg. It is sometimes possible for the material contained within the vial of "Epithelial Neutrophil-Activating Protein 78, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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