Granulocyte Colony Stimulating Factor Active Protein | Csf3 active protein

Mouse Granulocyte Colony Stimulating Factor Recombinant

Cat. Num. AAA76390

• Gene Names: Csf3; Csfg; G-CSF; MGI-IG
• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Granulocyte Colony Stimulating Factor; N/A; Mouse Granulocyte Colony Stimulating Factor Recombinant; Csf3 active protein

• Host: E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: IRecombinant mouse G-CSF was lyophilized from a 0.2 um filtered PBS solution.
• Sequence: MAQLSAQRRM KLMALQLLLW QSALWSGREA VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

• Source: Optimized DNA sequence encoding mouse Granulocyte Colony Stimulating Factor mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Biological Activity: The ED(50) was determined by the dose-dependent proliferation of murine M-NFS-60 cells is <.05 ng/ml, corresponding to a specific activity of > x units/mg.
• Method: Bone marrow transductionRetroviral transduction of bone marrow was done as previously described (6/ml) were prestimulated for 48 hours in IDMEM containing 10% Premium FBS, 2% Penicillin/Streptomycin, and 100 ng/ml each of recombinant murine TPO, G-CSF, and SCF.; For primary transduction, cells were resuspended in appropriate retroviral supernatant containing growth factors and seeded onto fibronectin coated wells Cell culture and retroviral transductionKasumi-1, 293T and U937 cells were cultured as previously described. G-CSF (granulocyte colony-stimulating factor)-mobilized peripheral blood CD34+ cells were cultured in Iscove's medium'>modified medium'>Dulbecco's medium supplemented with 20% fetal calf serum, 20?ng/ml Flt-3?l, 20?ng/ml GM-CSF, 20?ng/ml stem cell factor (SCF), 20?ng/ml thrombopoietin, 20?ng/ml interleukin (IL)-6, 10?ng/ml IL-3 (all cytokines were obtained,,), 100?U/ml penicillin/streptomycin and 2?mM L-glutamine.; Retroviral transduction and long-term cultivation were performed as previously described. IMR-90 cells (ATCC-CCL-186) were cultured as suggested by the supplier.
• Molecular Function: Cytokine
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

(Generation of conditional TAK1-deficient mice.Colony formation by BM cells from Map3k7)

Application Data

(G-CSF production by various mouse tumor models.Sera G-CSF levels determined from nontumor-bearing mice (n?=?10), mice treated with recombinant G-CSF protein (10 ug daily for 5 consecutive days) (n?=?8) or mice bearing 4T1 cells (n?=?9) or AT-3 tumor cells pooled from two separate experiments (n?=?9; overall tumor volume ?2cm3).)

Application Data

(Inhibition of neutrophil differentiation by IRF4.Wright-Giemsa staining of 32Dcl.3 cells transduced with empty MSCV-puro, MSCV-IRF4DYKDDDDK-puro or MSCV-IRF8DYKDDDDK-puro and cultured in the presence of IL-3 (upper panels) or G-CSF (for 7 days, lower panels).)

Product Categories/Family for Csf3 active protein

Hematopoietins; IL-6 gp130 family; Recombinant Mouse Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 6753536
• NCBI GeneID: 12985
• NCBI Accession #: NP_034101.1
• NCBI GenBank Nucleotide #: NM_009971.1
• UniProt Accession #: P09920; Q99062 CSF3R_HUMAN
• Molecular Weight: 22,421 Da
• NCBI Official Full Name: granulocyte colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: Csf3
• NCBI Official Synonym Symbols: Csfg; G-CSF; MGI-IG
• NCBI Protein Information: granulocyte colony-stimulating factor
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: Csf3
• UniProt Synonym Gene Names: Csfg; G-CSF
• UniProt Entry Name: CSF3_MOUSE