EGFR recombinant protein

EGFR Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA257396

• Gene Names: EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• Purity: >97 % as determined by SDS-PAGE.; >85% as determined by SEC-HPLC
• Synonyms: EGFR; N/A; EGFR Protein, Human, Recombinant (ECD, hFc Tag); Human EGFR/HER1/ErbB1 Protein (Fc Tag); epidermal growth factor receptor; ERBB Protein, Human; ERBB1 Protein, Human; HER1 Protein, Human; mENA Protein, Human; NISBD2 Protein, Human; PIG61 Protein, Human; EGFR recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97 % as determined by SDS-PAGE.; >85% as determined by SEC-HPLC
• Form/Format: Lyophilized from sterile PBS, pH 7.4; Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 860aa
• Sequence: Met1-Ser645

• Species: Human
• Tag: hFc
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu25
• Protein Construction: |A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was fused with the Fc region of human IgG1 at the C-terminus
• Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 10ug/ml (100ul/well) can bind human EGFR with a linear range of 0.64-400ng/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized recombinant human EGF at 10 ug/ml (100 ul/well) can bind human EGFR with a linear range of 0.64-400 ng/ml.)

Application Data

Related Product Information for EGFR recombinant protein

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-alpha, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo-or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Product Categories/Family for EGFR recombinant protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs); EGFR Signaling Pathway

References

Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.

NCBI and Uniprot Product Information

• NCBI GI #: 29725609
• NCBI GeneID: 1956
• NCBI Accession #: NP_005219
• NCBI GenBank Nucleotide #: NM_005228.4
• UniProt Accession #: P00533; O00688; O00732; P06268; Q14225; Q68GS5; Q92795; Q9BZS2; Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7
• Molecular Weight: The recombinant human EGFR/Fc chimera is a homodimeric protein. The reduced monomer consists of 860 amino acids and has a calculated molecular mass of 95 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110-140 kDa p
• NCBI Official Full Name: epidermal growth factor receptor isoform a
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: EGFR
• NCBI Official Synonym Symbols: ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• UniProt Gene Name: EGFR
• UniProt Synonym Gene Names: ERBB; ERBB1; HER1
• UniProt Entry Name: EGFR_HUMAN