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ELISA Kits

AAA Biotech (AAA Bio or AAABio) provides ready-to-use Enzyme-Linked Immunosorbent Assay (ELISA) kits. These kits are used to detect and quantify a variety of antigens, proteins, peptides, hormones, cytokines, and other biomarkers. Our kits can be used with a variety of biological samples, such as serum, plasma, tissue homogenates, cell culture lysates, and supernatants.


Viewing 5500-5550 of 115293 product results


product-image-AAA189012_SC15.jpg Standard Curve (Sample)

Hyaluronidase (HAase), CLIA Kit (Cat# AAA189012)

No reviews yet
Full Name
Human Hyaluronidase (HAase) CLIA Kit
Gene Names
HYAL1; MPS9; NAT6; LUCA1; HYAL-1
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189014_SC15.jpg Standard Curve (Sample)

Cytokeratin Fragment Antigen 21-1 (CYFRA21-1), CLIA Kit (Cat# AAA189014)

No reviews yet
Full Name
Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) CLIA Kit
Gene Names
KRT19; K19; CK19; K1CS
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189017_SC15.jpg Standard Curve (Sample)

Pleiotrophin (PTN), CLIA Kit (Cat# AAA189017)

No reviews yet
Full Name
Human Pleiotrophin (PTN) CLIA Kit
Gene Names
PTN; HARP; HBBM; HBNF; HBGF8; NEGF1; OSF-1; HB-GAM; HBGF-8; HBNF-1
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189018_SC15.jpg Standard Curve (Sample)

Thymic Stromal Lymphopoietin (TSLP), CLIA Kit (Cat# AAA189018)

No reviews yet
Full Name
Human Thymic Stromal Lymphopoietin (TSLP) CLIA Kit
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189020_SC15.jpg Standard Curve (Sample)

Serpin B3 (SERPINB3), CLIA Kit (Cat# AAA189020)

No reviews yet
Full Name
Human Serpin B3 (SERPINB3) CLIA Kit
Gene Names
SERPINB3; SCC; T4-A; SCCA1; SSCA1; SCCA-1; HsT1196; SCCA-PD
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189021_SC15.jpg Standard Curve (Sample)

Neutrophil gelatinase-associated lipocalin (NGAL), CLIA Kit (Cat# AAA189021)

No reviews yet
Full Name
Human Neutrophil gelatinase-associated lipocalin (NGAL) CLIA Kit
Gene Names
LCN2; p25; 24p3; MSFI; NGAL
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189030_SC15.jpg Standard Curve (Sample)

Myostatin (MSTN), CLIA Kit (Cat# AAA189030)

No reviews yet
Full Name
Human Myostatin (MSTN) CLIA Kit
Gene Names
MSTN; GDF8; MSLHP
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189032_SC15.jpg Standard Curve (Sample)

Insulin Like Growth Factor Binding Protein 7 (IGFBP7), CLIA Kit (Cat# AAA189032)

No reviews yet
Full Name
Human Insulin Like Growth Factor Binding Protein 7 (IGFBP7) CLIA Kit
Gene Names
IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189033_SC15.jpg Standard Curve (Sample)

Interleukin 21 (IL21), CLIA Kit (Cat# AAA189033)

No reviews yet
Full Name
Human Interleukin 21 (IL21) CLIA Kit
Gene Names
IL21; Za11; IL-21; CVID11
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189036_SC15.jpg Standard Curve (Sample)

Interleukin 28A (IL28A), CLIA Kit (Cat# AAA189036)

No reviews yet
Full Name
Human Interleukin 28A (IL28A) CLIA Kit
Gene Names
IFNL2; IL28A; IL-28A
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189044_SC15.jpg Standard Curve (Sample)

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1), CLIA Kit (Cat# AAA189044)

No reviews yet
Full Name
Human Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) CLIA Kit
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189050_SC15.jpg Standard Curve (Sample)

Kallikrein 7 (KLK7), CLIA Kit (Cat# AAA189050)

No reviews yet
Full Name
Human Kallikrein 7 (KLK7) CLIA Kit
Gene Names
KLK7; hK7; SCCE; PRSS6
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189052_SC15.jpg Standard Curve (Sample)

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), CLIA Kit (Cat# AAA189052)

No reviews yet
Full Name
Human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) CLIA Kit
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189053_SC15.jpg Standard Curve (Sample)

Epiregulin (EREG), CLIA Kit (Cat# AAA189053)

No reviews yet
Full Name
Human Epiregulin (EREG) CLIA Kit
Gene Names
EREG; ER; Ep; EPR
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189058_SC15.jpg Standard Curve (Sample)

Interleukin 33 (IL33), CLIA Kit (Cat# AAA189058)

No reviews yet
Full Name
Human Interleukin 33 (IL33) CLIA Kit
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189060_SC15.jpg Standard Curve (Sample)

Fatty Acid Binding Protein 5 (FABP5), CLIA Kit (Cat# AAA189060)

No reviews yet
Full Name
Human Fatty Acid Binding Protein 5 (FABP5) CLIA Kit
Gene Names
FABP5; EFABP; KFABP; E-FABP; PAFABP; PA-FABP
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189062_SC15.jpg Standard Curve (Sample)

Apolipoprotein B (APOB), CLIA Kit (Cat# AAA189062)

No reviews yet
Full Name
Human Apolipoprotein B (APOB) CLIA Kit
Gene Names
APOB; FLDB; FCHL2; LDLCQ4; apoB-48; apoB-100
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189066_SC15.jpg Standard Curve (Sample)

Creatine Kinase B (CK-BB), CLIA Kit (Cat# AAA189066)

No reviews yet
Full Name
Human Creatine Kinase B (CK-BB) CLIA Kit
Gene Names
CKB; BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189074_SC15.jpg Standard Curve (Sample)

VEGF Co Regulated Chemokine 1 (VCC1), CLIA Kit (Cat# AAA189074)

No reviews yet
Full Name
Rat VEGF Co Regulated Chemokine 1 (VCC1) CLIA Kit
Reactivity
Rat
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189078_SC15.jpg Standard Curve (Sample)

Growth Differentiation Factor 11 (GDF11), CLIA Kit (Cat# AAA189078)

No reviews yet
Full Name
Human Growth Differentiation Factor 11 (GDF11) CLIA Kit
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189083_SC15.jpg Standard Curve (Sample)

Anterior Gradient 2 (AGR2), CLIA Kit (Cat# AAA189083)

No reviews yet
Full Name
Human Anterior Gradient 2 (AGR2) CLIA Kit
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189087_SC15.jpg Standard Curve (Sample)

Cardiotrophin Like Cytokine Factor 1 (CLCF1), CLIA Kit (Cat# AAA189087)

No reviews yet
Full Name
Human Cardiotrophin Like Cytokine Factor 1 (CLCF1) CLIA Kit
Gene Names
CLCF1; CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189088_SC15.jpg Standard Curve (Sample)

Ferroportin (FPN), CLIA Kit (Cat# AAA189088)

No reviews yet
Full Name
Mouse Ferroportin (FPN) CLIA Kit
Reactivity
Mouse
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189090_SC15.jpg Standard Curve (Sample)

Phosphoserine Phosphatase (PSPH), CLIA Kit (Cat# AAA189090)

No reviews yet
Full Name
Human Phosphoserine Phosphatase (PSPH) CLIA Kit
Gene Names
PSPH; PSP; PSPHD
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189093_SC15.jpg Standard Curve (Sample)

Fibroblast Growth Factor 13 (FGF13), CLIA Kit (Cat# AAA189093)

No reviews yet
Full Name
Human Fibroblast Growth Factor 13 (FGF13) CLIA Kit
Gene Names
FGF13; FGF2; FHF2; FHF-2; FGF-13; LINC00889
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189098_SC15.jpg Standard Curve (Sample)

Troponin T Type 2, Cardiac (TNNT2), CLIA Kit (Cat# AAA189098)

No reviews yet
Full Name
Human Troponin T Type 2, Cardiac (TNNT2) CLIA Kit
Gene Names
TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189120_SC15.jpg Standard Curve (Sample)

Isocitrate Dehydrogenase 1, Soluble (IDH1), CLIA Kit (Cat# AAA189120)

No reviews yet
Full Name
Human Isocitrate Dehydrogenase 1, Soluble (IDH1) CLIA Kit
Gene Names
IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189124_SC15.jpg Standard Curve (Sample)

Cartilage Intermediate Layer Protein 2 (CILP2), CLIA Kit (Cat# AAA189124)

No reviews yet
Full Name
Human Cartilage Intermediate Layer Protein 2 (CILP2) CLIA Kit
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189131_SC15.jpg Standard Curve (Sample)

High Temperature Requirement Factor A1 (HTRA1), CLIA Kit (Cat# AAA189131)

No reviews yet
Full Name
Human High Temperature Requirement Factor A1 (HTRA1) CLIA Kit
Gene Names
HTRA1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189132_SC15.jpg Standard Curve (Sample)

Interleukin 1 Family, Member 9 (IL1F9), CLIA Kit (Cat# AAA189132)

No reviews yet
Full Name
Human Interleukin 1 Family, Member 9 (IL1F9) CLIA Kit
Gene Names
IL36G; IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189136_SC15.jpg Standard Curve (Sample)

Wingless Type MMTV Integration Site Family, Member 1 (WNT1), CLIA Kit (Cat# AAA189136)

No reviews yet
Full Name
Human Wingless Type MMTV Integration Site Family, Member 1 (WNT1) CLIA Kit
Gene Names
WNT1; INT1; OI15; BMND16
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189138_SC15.jpg Standard Curve (Sample)

Pim-3 Oncogene (PIM3), CLIA Kit (Cat# AAA189138)

No reviews yet
Full Name
Human Pim-3 Oncogene (PIM3) CLIA Kit
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189139_SC15.jpg Standard Curve (Sample)

Chemokine Like Factor Superfamily 6 (CKLFSF6), CLIA Kit (Cat# AAA189139)

No reviews yet
Full Name
Human Chemokine Like Factor Superfamily 6 (CKLFSF6) CLIA Kit
Gene Names
CMTM6; CKLFSF6; PRO2219
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189146_SC15.jpg Standard Curve (Sample)

Wingless Type MMTV Integration Site Family, Member 11 (WNT11), CLIA Kit (Cat# AAA189146)

No reviews yet
Full Name
Human Wingless Type MMTV Integration Site Family, Member 11 (WNT11) CLIA Kit
Gene Names
WNT11; HWNT11
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA189154_SC15.jpg Standard Curve (Sample)

Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), ELISA Kit (Cat# AAA189154)

No reviews yet
Full Name
Rat Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) Wide-range ELISA Kit
Reactivity
Rat
Applications
ELISA
Pricing
product-image-AAA189156_SC15.jpg Standard Curve (Sample)

Collagen Type III (COL3), ELISA Kit (Cat# AAA189156)

No reviews yet
Full Name
Human Collagen Type III (COL3) Wide-range ELISA Kit
Reactivity
Human
Applications
ELISA
Pricing
product-image-AAA189159_SC15.jpg Standard Curve (Sample)

Gelsolin (GSN), ELISA Kit (Cat# AAA189159)

No reviews yet
Full Name
Human Gelsolin (GSN) Wide-range ELISA Kit
Gene Names
GSN; ADF; AGEL
Reactivity
Human
Applications
ELISA
Pricing
product-image-AAA189161_SC15.jpg Standard Curve (Sample)

Complement Component 3a (C3a), ELISA Kit (Cat# AAA189161)

No reviews yet
Full Name
Human Complement Component 3a (C3a) Wide-range ELISA Kit
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
Reactivity
Human
Applications
ELISA
Pricing
product-image-AAA189167_SC15.jpg Standard Curve (Sample)

Ferritin (FE), ELISA Kit (Cat# AAA189167)

No reviews yet
Full Name
Rat Ferritin (FE) Wide-range ELISA Kit
Reactivity
Rat
Applications
ELISA
Pricing
product-image-AAA189172_SC15.jpg Standard Curve (Sample)

Apolipoprotein B100 (APOB100), ELISA Kit (Cat# AAA189172)

No reviews yet
Full Name
Rat Apolipoprotein B100 (APOB100) Wide-range ELISA Kit
Gene Names
Apob; Aa1064; Ac1-060; ApoB-48; ApoB-100; Apo B-100
Reactivity
Rat
Applications
ELISA
Pricing
product-image-AAA189175_SC15.jpg Standard Curve (Sample)

Macrophage Migration Inhibitory Factor (MIF), ELISA Kit (Cat# AAA189175)

No reviews yet
Full Name
Rat Macrophage Migration Inhibitory Factor (MIF) Wide-range ELISA Kit
Reactivity
Rat
Applications
ELISA
Pricing
product-image-AAA189176_SC15.jpg Standard Curve (Sample)

Complement 1q (C1q), ELISA Kit (Cat# AAA189176)

No reviews yet
Full Name
Mouse Complement 1q (C1q) Wide-range ELISA Kit
Reactivity
Mouse
Applications
ELISA
product-image-AAA189180_SC15.jpg Standard Curve (Sample)

Pulmonary Surfactant Associated Protein A1 (SFTPA1), ELISA Kit (Cat# AAA189180)

No reviews yet
Full Name
Human Pulmonary Surfactant Associated Protein A1 (SFTPA1) Wide-range ELISA Kit
Gene Names
SFTPA1; SPA; PSAP; PSPA; SP-A; SPA1; PSP-A; SFTP1; SP-A1; COLEC4; SFTPA1B; SP-A1 beta; SP-A1 delta; SP-A1 gamma; SP-A1 epsilon
Reactivity
Human
Applications
ELISA
Pricing
product-image-AAA189183_SC15.jpg Standard Curve (Sample)

Amyloid Precursor Protein (APP), ELISA Kit (Cat# AAA189183)

No reviews yet
Full Name
Human Amyloid Precursor Protein (APP) Wide-range ELISA Kit
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity
Human
Applications
ELISA
Pricing
product-image-AAA188888_SC15.jpg Standard Curve (Sample)

Platelet Factor 4 (PF4), CLIA Kit (Cat# AAA188888)

No reviews yet
Full Name
Human Platelet Factor 4 (PF4) CLIA Kit
Gene Names
PF4; PF-4; CXCL4; SCYB4
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA188896_SC15.jpg Standard Curve (Sample)

Alpha-1-Microglobulin (a1M), CLIA Kit (Cat# AAA188896)

No reviews yet
Full Name
Human Alpha-1-Microglobulin (a1M) CLIA Kit
Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA188900_SC15.jpg Standard Curve (Sample)

Poly ADP Ribose Polymerase (PARP), CLIA Kit (Cat# AAA188900)

No reviews yet
Full Name
Human Poly ADP Ribose Polymerase (PARP) CLIA Kit
Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA188903_SC15.jpg Standard Curve (Sample)

Galectin 1 (GAL1), CLIA Kit (Cat# AAA188903)

No reviews yet
Full Name
Human Galectin 1 (GAL1) CLIA Kit
Gene Names
LGALS1; GBP; GAL1
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA188904_SC15.jpg Standard Curve (Sample)

Thyroglobulin (TG), CLIA Kit (Cat# AAA188904)

No reviews yet
Full Name
Human Thyroglobulin (TG) CLIA Kit
Gene Names
TG; TGN; AITD3
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing
product-image-AAA188911_SC15.jpg Standard Curve (Sample)

High Mobility Group Protein 1 (HMGB1), CLIA Kit (Cat# AAA188911)

No reviews yet
Full Name
Human High Mobility Group Protein 1 (HMGB1) CLIA Kit
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Reactivity
Human
Applications
Chemiluminescence Immunoassay
Pricing

Our validated kits are trusted by researchers worldwide for their precision, reliability, and ease of use. Researchers take advantage of our high-quality kits for studying biochemical pathways, disease monitoring and progressions, pathogen detection, pharmacological testing, biomarker discovery, etc.


However, if you can't find the target or species you need for your research, don’t worry; we provide Custom Assay development services as well. So, all you need to do is submit your request, and we will determine if we can design an ELISA testing kit as per your specifications.


Our catalog contains ELISA assay kits that are reactive with samples coming from a very wide range of different species, including:


  • Human
  • Mouse
  • Rat
  • Bovine
  • Rabbit
  • Chicken
  • Porcine
  • Monkey
  • Goat
  • Sheep
  • Canine
  • Guinea Pig
  • And more…

We also have kits that are compatible with samples originating from less common species, including:


  • Horse/Equine
  • Fish
  • Cat
  • Hamster
  • Donkey
  • Duck
  • Pigeon
  • Camel
  • Goose
  • Plant
  • And more…

Check out a comprehensive catalog of ready-to-use assay kits and place your order for ELISA kits online now! Want to learn more about ELISA? Here is our complete guide to ELISA.


What are the Benefits of ELISA Kits?


  • High sensitivity and specificity: ELISA is capable of detecting even low levels of analytes with excellent accuracy. Learn more in our guide on ELISA sensitivity and specificity.

  • Quantitative results: Provides precise antigen and antibody concentration results.

  • Scalable and high throughput: Ideal for analyzing large numbers of samples at the same time.

  • Easy to use: Intuitive and straightforward procedure.

  • Versatile applications: Ideal for use in immunology, cancer research, infectious disease, food safety, and more.


What is Included in an ELISA Kit?


Each ELISA kit from AAA Biotech typically contains the following components:


  • Pre-coated 96/48/24-well microplate

  • Standards and/or controls

  • Detection antibody

  • Enzyme conjugate

  • Substrate solution (e.g., TMB)

  • Stop solution

  • Sample diluent and wash buffer

  • Detailed protocol and instructions

Why Buy ELISA Kits from AAA Biotech?


  • Quick and Convenient Ordering: Simple online ordering process with responsive customer support.

  • Wide Selection of ELISA Kits: Extensive product range covering wide range of species, targets, and research areas.

  • Affordable Prices: High-quality kits at competitive prices to support all research budgets.

  • Over 10 Years Supporting the Research Community: A dedicated partner to researchers worldwide.

  • Shipping to 100+ Countries: Reliable international delivery with tracked shipments.

  • High-Quality, Validated Reagents: Designed for accuracy, reproducibility, and performance in research settings.

  • Trusted by Researchers Globally: Used in universities, biotech labs, and research institutes around the world.

  • Various Applications: Ideal for basic science, immunology, oncology, infectious disease study, and more.

When to Choose a Custom ELISA Kit, When to Buy a Ready-to-Use ELISA Kit?


Requirements for an ELISA kit can vary from project-to-project. Here are some strategies for making an informed decision about whether to go with a ready-to-use or a customized ELISA kit:


When to Choose a Ready-to-Use ELISA Kit:


  • Your target analyte is already available in the catalog

  • You need quick and reliable results without additional optimization

  • You are using “typical” sample types like serum, plasma, or cell culture supernatants

  • Your project requires cost-effective, validated, and widely used assays


When to Choose a Custom ELISA Kit:


  • Your analyte of interest is rare, novel, or not covered in existing kits

  • You are working with uncommon species or non-standard sample types

  • You need a specific assay format (competitive, sandwich, or indirect) tailored to your study

  • Your research demands unique sensitivity, specificity, or detection ranges

  • You want the assay optimized for your lab’s workflow and requirements


AAA Biotech supports both ready-to-use ELISA kits and Custom Assay Development Services, giving researchers the flexibility to select the best solution for their scientific goals.


ELISA Kit Protocol


The user manuals for our ELISA kits are conveniently accessible through the datasheets on our website or can be provided upon request. Each manual contains a clear explanation of the assay principle, a complete list of kit components, and a step-by-step protocol. These guides are designed to be straightforward, making them suitable for researchers who are new to ELISA techniques as well as experienced users who need a quick, reliable reference. In addition, Safety Data Sheets (SDS) for all products are available upon request to ensure safe handling.


What sets AAA Biotech apart is not just the range of assays we offer, but the consistency and reliability of our reagents. Every kit is carefully validated, ensuring that researchers can trust the results for both routine testing and advanced studies.


To avoid common errors and improve accuracy, refer to our guide on “common ELISA mistakes and how to avoid them”.


FAQ


1. When should I use a competitive ELISA assay?


Competitive ELISA is ideal when the target analyte is a smaller molecule (such as hormones or other short peptides or lipids/sugars) and there is unlikely to be sufficient surface or possible epitopes on the analyte/molecule to allow for two antibodies to be able to bind to it simultaneously (as would be required by an ELISA using the traditional “sandwich” design format).


2. What samples can ELISA kits be used for?


ELISA kits are compatible with a wide variety of biological samples, including serum, plasma, cell culture supernatants, urine, saliva, and tissue homogenates. Serum & plasma are routinely tested with the vast majority of kits in our catalog, but since many target analytes do not exist in these sample types, some will be further validated with various tissue homogenate or cell lysate samples. The samples ultimately tested by the manufacturing lab technician will largely depend on the target analyte’s typical location in an organism, or where its presence is of interest to the research community.


3. How long can I store my sample before using it for an ELISA assay?


Sample shelf-life ultimately depends on the intrinsic stability of the target analyte in the samples’ specific biological matrix, and also any other factors affecting it during storage (such as added preservatives and temperature). Generally:


  • Short-term (up to 48 hours): Store at 2–8°C
  • Long-term: Store at -20°C or -80°C for most biological samples

4. Where can I buy an ELISA test kit?


You can browse AAA Biotech’s comprehensive catalog and place your order directly through our website. For special requirements, you can also submit a request for a custom ELISA kit.


5. Why are ELISA tests costly?


ELISA kits involve high-quality reagents, validated antibodies, and precise controls to ensure accurate and reproducible results. Costs also reflect research and development, quality control, and the rigorous validation process required to support reliable scientific outcomes. While they may seem expensive, the investment ensures high sensitivity, specificity, and trustworthy data required by our users’ critical research.


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