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ELISA Kits

AAA Biotech (AAA Bio or AAABio) provides ready-to-use Enzyme-Linked Immunosorbent Assay (ELISA) kits. These kits are used to detect and quantify a variety of antigens, proteins, peptides, hormones, cytokines, and other biomarkers. Our kits can be used with a variety of biological samples, such as serum, plasma, tissue homogenates, cell culture lysates, and supernatants.


Viewing 800-850 of 130489 product results


product-image-AAA71158_SC15.jpg Standard Curve (Sample)

Hypoxia Inducible Factor 2 Alpha (HIF2a), RTU ELISA Kit (Cat# AAA71158)

No reviews yet
Full Name
Human Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit
Gene Names
EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
Reactivity
Human
Pricing
product-image-AAA71183_SC15.jpg Standard Curve (Sample)

Heterogeneous Nuclear Ribonucleoprotein K (HNRPK), RTU ELISA Kit (Cat# AAA71183)

No reviews yet
Full Name
Human Heterogeneous Nuclear Ribonucleoprotein K (HNRPK) ELISA Kit
Gene Names
HNRNPK; AUKS; CSBP; TUNP; HNRPK
Reactivity
Human
Pricing
product-image-AAA71197_SC15.jpg Standard Curve (Sample)

Hermansky Pudlak Syndrome Protein 6 (HPS6), RTU ELISA Kit (Cat# AAA71197)

No reviews yet
Full Name
Human Hermansky Pudlak Syndrome Protein 6 (HPS6) ELISA Kit
Gene Names
HPS6; BLOC2S3
Reactivity
Human
Pricing
product-image-AAA71227_SC15.jpg Standard Curve (Sample)

17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3), RTU ELISA Kit (Cat# AAA71227)

No reviews yet
Full Name
Human 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) ELISA Kit
Gene Names
HSD17B3; EDH17B3; SDR12C2
Reactivity
Human
Pricing
product-image-AAA71236_SC15.jpg Standard Curve (Sample)

Heat Shock Protein 47 (HSP47), RTU ELISA Kit (Cat# AAA71236)

No reviews yet
Full Name
Human Heat Shock Protein 47 (HSP47) ELISA Kit
Gene Names
SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
Reactivity
Human
Pricing
product-image-AAA71211_SC15.jpg Standard Curve (Sample)

Histamine Receptor H1 (HRH1), RTU ELISA Kit (Cat# AAA71211)

No reviews yet
Full Name
Rat Histamine Receptor H1 (HRH1) ELISA Kit
Gene Names
Hrh1; Hisr
Reactivity
Rat
Pricing
product-image-AAA71214_SC15.jpg Standard Curve (Sample)

Histamine Receptor H4 (HRH4), RTU ELISA Kit (Cat# AAA71214)

No reviews yet
Full Name
Mouse Histamine Receptor H4 (HRH4) ELISA Kit
Gene Names
Hrh4; H4; H4R; BG26; HH4R; AXOR35; GPRv53; GPCR105
Reactivity
Mouse
Pricing
product-image-AAA71216_SC15.jpg Standard Curve (Sample)

Hornerin (HRNR), RTU ELISA Kit (Cat# AAA71216)

No reviews yet
Full Name
Human Hornerin (HRNR) ELISA Kit
Gene Names
HRNR; FLG3; S100A16; S100a18
Reactivity
Human
Pricing
product-image-AAA66119_SC15.jpg Standard Curve (Sample)

Adiponectin Receptor 1 (ADIPOR1), ELISA Kit (Cat# AAA66119)

No reviews yet
Full Name
Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit
Reactivity
Rat
Pricing
product-image-AAA66128_SC15.png Standard Curve (Sample)

Macrophage Inflammatory Protein 1 Alpha (MIP1a), ELISA Kit (Cat# AAA66128)

No reviews yet
Full Name
Rat Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit
Gene Names
Ccl3; Scya3; MIP-1a
Reactivity
Rat
Pricing
product-image-AAA66137_SC15.png Standard Curve (Sample)

Heme Oxygenase 1, Decycling (HO1), ELISA Kit (Cat# AAA66137)

No reviews yet
Full Name
Mouse Heme Oxygenase 1, Decycling (HO1) ELISA Kit
Gene Names
Hmox1; HO1; HO-1; Hmox; Hemox; Hsp32; D8Wsu38e
Reactivity
Mouse
Pricing
product-image-AAA66141_SC15.jpg Standard Curve (Sample)

Phosphatase And Tensin Homolog (PTEN), ELISA Kit (Cat# AAA66141)

No reviews yet
Full Name
Mouse Phosphatase And Tensin Homolog (PTEN) ELISA Kit
Gene Names
Pten; TEP1; MMAC1; AI463227; 2310035O07Rik; A130070J02Rik; B430203M17Rik
Reactivity
Mouse
Pricing
product-image-AAA66151_SC15.png Standard Curve (Sample)

Sphingosine 1 Phosphate Receptor 1 (S1PR1), ELISA Kit (Cat# AAA66151)

No reviews yet
Full Name
Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit
Gene Names
S1pr1; S1p; Edg1; Lpb1; S1p1; AI849002
Reactivity
Mouse
Pricing
product-image-AAA66159_SC15.jpg Standard Curve (Sample)

Chloride Channel Accessory 1 (CLCA1), ELISA Kit (Cat# AAA66159)

No reviews yet
Full Name
Human Chloride Channel Accessory 1 (CLCA1) ELISA Kit
Gene Names
CLCA1; CACC; GOB5; CACC1; CLCRG1; CaCC-1; hCLCA1; hCaCC-1
Reactivity
Human
Pricing
product-image-AAA66163_SC15.jpg Standard Curve (Sample)

Laminin Alpha 4 (LAMa4), ELISA Kit (Cat# AAA66163)

No reviews yet
Full Name
Human Laminin Alpha 4 (LAMa4) ELISA Kit
Gene Names
LAMA4; LAMA3; CMD1JJ; LAMA4*-1
Reactivity
Human
Pricing
product-image-AAA66171_SC15.jpg Standard Curve (Sample)

Interleukin 4 (IL4), ELISA Kit (Cat# AAA66171)

No reviews yet
Full Name
Rabbit Interleukin 4 (IL4) ELISA Kit
Gene Names
IL4; IL2; BSF-1
Reactivity
Rabbit
Pricing
product-image-AAA66176_SC15.jpg Standard Curve (Sample)

G Protein Beta 1 (GNb1), ELISA Kit (Cat# AAA66176)

No reviews yet
Full Name
Mouse G Protein Beta 1 (GNb1) ELISA Kit
Gene Names
Gnb1; Gnb-1; C77571; AA409223
Reactivity
Mouse
Pricing
product-image-AAA66177_SC15.jpg Standard Curve (Sample)

Renalase (RNLS), ELISA Kit (Cat# AAA66177)

No reviews yet
Full Name
Human Renalase (RNLS) ELISA Kit
Gene Names
RNLS; C10orf59; RENALASE
Reactivity
Human
Pricing
product-image-AAA66183_SC15.png Standard Curve (Sample)

Splicing Factor 3B Subunit 3 (SF3B3), ELISA Kit (Cat# AAA66183)

No reviews yet
Full Name
Mouse Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit
Gene Names
Sf3b3; RSE1; SAP130; AA409318; mKIAA0017; D8Ertd633e; 1810061H24Rik; 5730409A01Rik
Reactivity
Mouse
Pricing
product-image-AAA66188_SC15.jpg Standard Curve (Sample)

Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2), ELISA Kit (Cat# AAA66188)

No reviews yet
Full Name
Human Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) ELISA Kit
Gene Names
CPT2; CPT1; IIAE4; CPTASE
Reactivity
Human
Pricing
product-image-AAA66190_SC15.jpg Standard Curve (Sample)

Interferon Gamma (IFNg), ELISA Kit (Cat# AAA66190)

No reviews yet
Full Name
Guinea pig Interferon Gamma (IFNg) ELISA Kit
Reactivity
Guinea Pig
Pricing
product-image-AAA66201_SC15.jpg Standard Curve (Sample)

Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1), ELISA Kit (Cat# AAA66201)

No reviews yet
Full Name
Human Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) ELISA Kit
Gene Names
CHRNA1; ACHRA; ACHRD; CHRNA; CMS1A; CMS1B; CMS2A; FCCMS; SCCMS
Reactivity
Human
Pricing
product-image-AAA66202_SC15.jpg Standard Curve (Sample)

Cytochrome P450 1B1 (CYP1B1), ELISA Kit (Cat# AAA66202)

No reviews yet
Full Name
Human Cytochrome P450 1B1 (CYP1B1) ELISA Kit
Gene Names
CYP1B1; CP1B; GLC3A; CYPIB1; P4501B1
Reactivity
Human
Pricing
product-image-AAA66215_SC15.jpg Standard Curve (Sample)

Transferrin (TRF), ELISA Kit (Cat# AAA66215)

No reviews yet
Full Name
Human Transferrin (TRF) ELISA Kit
Gene Names
TF; TFQTL1; PRO1557; PRO2086; HEL-S-71p
Reactivity
Human
Pricing
product-image-AAA66225_SC15.jpg Standard Curve (Sample)

Klotho (KL), ELISA Kit (Cat# AAA66225)

No reviews yet
Full Name
Mouse Klotho (KL) ELISA Kit
Gene Names
Kl; alpha-kl
Reactivity
Mouse
Pricing
product-image-AAA66230_SC15.jpg Standard Curve (Sample)

Elastin (ELN), ELISA Kit (Cat# AAA66230)

No reviews yet
Full Name
Rat Elastin (ELN) ELISA Kit
Gene Names
Eln; Trela; TREL11; Trela26; RATTREL11
Reactivity
Rat
Pricing
product-image-AAA66234_SC15.jpg Standard Curve (Sample)

Lactate Dehydrogenase B (LDHB), ELISA Kit (Cat# AAA66234)

No reviews yet
Full Name
Mouse Lactate Dehydrogenase B (LDHB) ELISA Kit
Gene Names
Ldhb; Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2; AI790582
Reactivity
Mouse
Pricing
product-image-AAA70352_SC15.jpg Standard Curve (Sample)

Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g), RTU ELISA Kit (Cat# AAA70352)

No reviews yet
Full Name
Human Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g) ELISA Kit
Gene Names
EEF1G; EF1G; GIG35
Reactivity
Human
Pricing
product-image-AAA70355_SC15.jpg Standard Curve (Sample)

Ephrin A3 (EFNA3), RTU ELISA Kit (Cat# AAA70355)

No reviews yet
Full Name
Human Ephrin A3 (EFNA3) ELISA Kit
Gene Names
EFNA3; EFL2; EPLG3; LERK3; Ehk1-L
Reactivity
Human
Pricing
product-image-AAA70362_SC15.jpg Standard Curve (Sample)

EGF Like Domain Protein, Multiple 7 (EGFL7), RTU ELISA Kit (Cat# AAA70362)

No reviews yet
Full Name
Mouse EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit
Gene Names
Egfl7; Zneu1; VE-statin
Reactivity
Mouse
Pricing
product-image-AAA70365_SC15.jpg Standard Curve (Sample)

Epidermal Growth Factor Receptor 2 (EGFR2), RTU ELISA Kit (Cat# AAA70365)

No reviews yet
Full Name
Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human
Pricing
product-image-AAA70367_SC15.jpg Standard Curve (Sample)

Epidermal Growth Factor Receptor 2 (EGFR2), RTU ELISA Kit (Cat# AAA70367)

No reviews yet
Full Name
Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit
Reactivity
Rat
Pricing
product-image-AAA70398_SC15.jpg Standard Curve (Sample)

Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1), RTU ELISA Kit (Cat# AAA70398)

No reviews yet
Full Name
Human Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) ELISA Kit
Gene Names
ELOVL1; Ssc1; CGI-88
Reactivity
Human
Pricing
product-image-AAA70403_SC15.jpg Standard Curve (Sample)

Echinoderm Microtubule Associated Protein Like Protein 2 (EML2), RTU ELISA Kit (Cat# AAA70403)

No reviews yet
Full Name
Human Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) ELISA Kit
Gene Names
EML2; ELP70; EMAP2; EMAP-2
Reactivity
Human
Pricing
product-image-AAA70407_SC15.jpg Standard Curve (Sample)

Epithelial Membrane Protein 3 (EMP3), RTU ELISA Kit (Cat# AAA70407)

No reviews yet
Full Name
Human Epithelial Membrane Protein 3 (EMP3) ELISA Kit
Gene Names
EMP3; YMP
Reactivity
Human
Pricing
product-image-AAA70409_SC15.jpg Standard Curve (Sample)

Engrailed Homeobox Protein 2 (EN2), RTU ELISA Kit (Cat# AAA70409)

No reviews yet
Full Name
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit
Reactivity
Human
Pricing
product-image-AAA70418_SC15.jpg Standard Curve (Sample)

Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), RTU ELISA Kit (Cat# AAA70418)

No reviews yet
Full Name
Human Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA Kit
Gene Names
ENPP1; M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
Reactivity
Human
Pricing
product-image-AAA70427_SC15.jpg Standard Curve (Sample)

Ephrin Type A Receptor 1 (EPHA1), RTU ELISA Kit (Cat# AAA70427)

No reviews yet
Full Name
Human Ephrin Type A Receptor 1 (EPHA1) ELISA Kit
Gene Names
EPHA1; EPH; EPHT; EPHT1
Reactivity
Human
Pricing
product-image-AAA70447_SC15.jpg Standard Curve (Sample)

Estrogen Receptor Alpha (ERa), RTU ELISA Kit (Cat# AAA70447)

No reviews yet
Full Name
Mouse Estrogen Receptor Alpha (ERa) ELISA Kit
Gene Names
Esr1; ER; ERa; ESR; Estr; Estra; Nr3a1; ERalpha; ER-alpha
Reactivity
Mouse
Pricing
product-image-AAA70450_SC15.jpg Standard Curve (Sample)

V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3), RTU ELISA Kit (Cat# AAA70450)

No reviews yet
Full Name
Human V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) ELISA Kit
Gene Names
ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
Reactivity
Human
Pricing
product-image-AAA70457_SC15.jpg Standard Curve (Sample)

Epiregulin (EREG), RTU ELISA Kit (Cat# AAA70457)

No reviews yet
Full Name
Human Epiregulin (EREG) ELISA Kit
Gene Names
EREG; ER; Ep; EPR
Reactivity
Human
Pricing
product-image-AAA70479_SC15.jpg Standard Curve (Sample)

Exosome Component 2 (EXOSC2), RTU ELISA Kit (Cat# AAA70479)

No reviews yet
Full Name
Human Exosome Component 2 (EXOSC2) ELISA Kit
Gene Names
EXOSC2; p7; RRP4; SHRF; Rrp4p; hRrp4p
Reactivity
Human
Pricing
product-image-AAA70482_SC15.jpg Standard Curve (Sample)

Enhancer Of Zeste Homolog 1 (EZH1), RTU ELISA Kit (Cat# AAA70482)

No reviews yet
Full Name
Human Enhancer Of Zeste Homolog 1 (EZH1) ELISA Kit
Gene Names
EZH1; KMT6B
Reactivity
Human
Pricing
product-image-AAA70494_SC15.jpg Standard Curve (Sample)

Coagulation Factor XIII B Polypeptide (F13B), RTU ELISA Kit (Cat# AAA70494)

No reviews yet
Full Name
Mouse Coagulation Factor XIII B Polypeptide (F13B) ELISA Kit
Gene Names
F13b; Cf13b; Cf-13b
Reactivity
Mouse
Pricing
product-image-AAA70496_SC15.jpg Standard Curve (Sample)

Coagulation Factor II (F2), RTU ELISA Kit (Cat# AAA70496)

No reviews yet
Full Name
Mouse Coagulation Factor II (F2) ELISA Kit
Gene Names
F2; Cf2; FII; Cf-2
Reactivity
Mouse
Pricing
product-image-AAA70576_SC15.jpg Standard Curve (Sample)

Fibrillin 1 (FBN1), RTU ELISA Kit (Cat# AAA70576)

No reviews yet
Full Name
Human Fibrillin 1 (FBN1) ELISA Kit
Gene Names
FBN1; FBN; SGS; WMS; MASS; MFLS; MFS1; OCTD; SSKS; WMS2; ACMICD; ECTOL1; GPHYSD2
Reactivity
Human
Pricing
product-image-AAA70581_SC15.jpg Standard Curve (Sample)

Receptor II For The Fc Region Of Immunoglobulin E (FceRII), RTU ELISA Kit (Cat# AAA70581)

No reviews yet
Full Name
Human Receptor II For The Fc Region Of Immunoglobulin E (FceRII) ELISA Kit
Gene Names
FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
Reactivity
Human
Pricing
product-image-AAA70588_SC15.jpg Standard Curve (Sample)

Receptor II For The Fc Region Of Immunoglobulin G (FcgRII), RTU ELISA Kit (Cat# AAA70588)

No reviews yet
Full Name
Human Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) ELISA Kit
Gene Names
FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Reactivity
Human
Pricing
product-image-AAA70590_SC15.jpg Standard Curve (Sample)

Fc Fragment Of IgG Receptor Transporter Alpha (FcgRT), RTU ELISA Kit (Cat# AAA70590)

No reviews yet
Full Name
Human Fc Fragment Of IgG Receptor Transporter Alpha (FcgRT) ELISA Kit
Gene Names
FCGRT; FCRN; alpha-chain
Reactivity
Human
Pricing
product-image-AAA70594_SC15.jpg Standard Curve (Sample)

Ficolin 2 (FCN2), RTU ELISA Kit (Cat# AAA70594)

No reviews yet
Full Name
Human Ficolin 2 (FCN2) ELISA Kit
Gene Names
FCN2; P35; FCNL; EBP-37; ficolin-2
Reactivity
Human
Pricing

Our validated kits are trusted by researchers worldwide for their precision, reliability, and ease of use. Researchers take advantage of our high-quality kits for studying biochemical pathways, disease monitoring and progressions, pathogen detection, pharmacological testing, biomarker discovery, etc.


However, if you can't find the target or species you need for your research, don’t worry; we provide Custom Assay development services as well. So, all you need to do is submit your request, and we will determine if we can design an ELISA testing kit as per your specifications.


Our catalog contains ELISA assay kits that are reactive with samples coming from a very wide range of different species, including:


  • Human
  • Mouse
  • Rat
  • Bovine
  • Rabbit
  • Chicken
  • Porcine
  • Monkey
  • Goat
  • Sheep
  • Canine
  • Guinea Pig
  • And more…

We also have kits that are compatible with samples originating from less common species, including:


  • Horse/Equine
  • Fish
  • Cat
  • Hamster
  • Donkey
  • Duck
  • Pigeon
  • Camel
  • Goose
  • Plant
  • And more…

Check out a comprehensive catalog of ready-to-use assay kits and place your order for ELISA kits online now! Want to learn more about ELISA? Here is our complete guide to ELISA.


What are the Benefits of ELISA Kits?


  • High sensitivity and specificity: ELISA is capable of detecting even low levels of analytes with excellent accuracy. Learn more in our guide on ELISA sensitivity and specificity.

  • Quantitative results: Provides precise antigen and antibody concentration results.

  • Scalable and high throughput: Ideal for analyzing large numbers of samples at the same time.

  • Easy to use: Intuitive and straightforward procedure.

  • Versatile applications: Ideal for use in immunology, cancer research, infectious disease, food safety, and more.


What is Included in an ELISA Kit?


Each ELISA kit from AAA Biotech typically contains the following components:


  • Pre-coated 96/48/24-well microplate

  • Standards and/or controls

  • Detection antibody

  • Enzyme conjugate

  • Substrate solution (e.g., TMB)

  • Stop solution

  • Sample diluent and wash buffer

  • Detailed protocol and instructions

Why Buy ELISA Kits from AAA Biotech?


  • Quick and Convenient Ordering: Simple online ordering process with responsive customer support.

  • Wide Selection of ELISA Kits: Extensive product range covering wide range of species, targets, and research areas.

  • Affordable Prices: High-quality kits at competitive prices to support all research budgets.

  • Over 10 Years Supporting the Research Community: A dedicated partner to researchers worldwide.

  • Shipping to 100+ Countries: Reliable international delivery with tracked shipments.

  • High-Quality, Validated Reagents: Designed for accuracy, reproducibility, and performance in research settings.

  • Trusted by Researchers Globally: Used in universities, biotech labs, and research institutes around the world.

  • Various Applications: Ideal for basic science, immunology, oncology, infectious disease study, and more.

When to Choose a Custom ELISA Kit, When to Buy a Ready-to-Use ELISA Kit?


Requirements for an ELISA kit can vary from project-to-project. Here are some strategies for making an informed decision about whether to go with a ready-to-use or a customized ELISA kit:


When to Choose a Ready-to-Use ELISA Kit:


  • Your target analyte is already available in the catalog

  • You need quick and reliable results without additional optimization

  • You are using “typical” sample types like serum, plasma, or cell culture supernatants

  • Your project requires cost-effective, validated, and widely used assays


When to Choose a Custom ELISA Kit:


  • Your analyte of interest is rare, novel, or not covered in existing kits

  • You are working with uncommon species or non-standard sample types

  • You need a specific assay format (competitive, sandwich, or indirect) tailored to your study

  • Your research demands unique sensitivity, specificity, or detection ranges

  • You want the assay optimized for your lab’s workflow and requirements


AAA Biotech supports both ready-to-use ELISA kits and Custom Assay Development Services, giving researchers the flexibility to select the best solution for their scientific goals.


ELISA Kit Protocol


The user manuals for our ELISA kits are conveniently accessible through the datasheets on our website or can be provided upon request. Each manual contains a clear explanation of the assay principle, a complete list of kit components, and a step-by-step protocol. These guides are designed to be straightforward, making them suitable for researchers who are new to ELISA techniques as well as experienced users who need a quick, reliable reference. In addition, Safety Data Sheets (SDS) for all products are available upon request to ensure safe handling.


What sets AAA Biotech apart is not just the range of assays we offer, but the consistency and reliability of our reagents. Every kit is carefully validated, ensuring that researchers can trust the results for both routine testing and advanced studies.


To avoid common errors and improve accuracy, refer to our guide on “common ELISA mistakes and how to avoid them”.


FAQ


1. When should I use a competitive ELISA assay?


Competitive ELISA is ideal when the target analyte is a smaller molecule (such as hormones or other short peptides or lipids/sugars) and there is unlikely to be sufficient surface or possible epitopes on the analyte/molecule to allow for two antibodies to be able to bind to it simultaneously (as would be required by an ELISA using the traditional “sandwich” design format).


2. What samples can ELISA kits be used for?


ELISA kits are compatible with a wide variety of biological samples, including serum, plasma, cell culture supernatants, urine, saliva, and tissue homogenates. Serum & plasma are routinely tested with the vast majority of kits in our catalog, but since many target analytes do not exist in these sample types, some will be further validated with various tissue homogenate or cell lysate samples. The samples ultimately tested by the manufacturing lab technician will largely depend on the target analyte’s typical location in an organism, or where its presence is of interest to the research community.


3. How long can I store my sample before using it for an ELISA assay?


Sample shelf-life ultimately depends on the intrinsic stability of the target analyte in the samples’ specific biological matrix, and also any other factors affecting it during storage (such as added preservatives and temperature). Generally:


  • Short-term (up to 48 hours): Store at 2–8°C
  • Long-term: Store at -20°C or -80°C for most biological samples

4. Where can I buy an ELISA test kit?


You can browse AAA Biotech’s comprehensive catalog and place your order directly through our website. For special requirements, you can also submit a request for a custom ELISA kit.


5. Why are ELISA tests costly?


ELISA kits involve high-quality reagents, validated antibodies, and precise controls to ensure accurate and reproducible results. Costs also reflect research and development, quality control, and the rigorous validation process required to support reliable scientific outcomes. While they may seem expensive, the investment ensures high sensitivity, specificity, and trustworthy data required by our users’ critical research.


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