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product-image-AAA76226_ELISA11.jpg ELISA (Biotinylated Human EpCAM/TROP1 Protein ELISA DataImmobilized Biotinylated Human EpCAM, His Tag at 0.2?g/ml (100ul/Well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.)

EpCAM/TROP1 recombinant protein

Biotinylated Human EpCAM/TROP1 Protein Recombinant

Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
EpCAM/TROP1; N/A; Biotinylated Human EpCAM/TROP1 Protein Recombinant; EpCAM/TROP1 recombinant protein
Ordering
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Human EpCAM/TROP1 was lyophilized from 0.2 um filtered PBS solution pH7.4
Source
DNA sequence encoding Human EpCAM/TROP1 including a C-His-Avi tag was expressed in HEK293 Cells.The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.

ELISA

(Biotinylated Human EpCAM/TROP1 Protein ELISA DataImmobilized Biotinylated Human EpCAM, His Tag at 0.2?g/ml (100ul/Well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.)

product-image-AAA76226_ELISA11.jpg ELISA (Biotinylated Human EpCAM/TROP1 Protein ELISA DataImmobilized Biotinylated Human EpCAM, His Tag at 0.2?g/ml (100ul/Well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.)

Application Data

(Biotinylated Human EpCAM/TROP1 Protein SEC-HPLCThe purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC.)

product-image-AAA76226_AD13.jpg Application Data (Biotinylated Human EpCAM/TROP1 Protein SEC-HPLCThe purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC.)

Tris-Bis-PAGE

(Biotinylated Human EpCAM/TROP1 Protein Tris-Bis PAGEBiotinylated Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..)

product-image-AAA76226_PAGE15.jpg Tris-Bis-PAGE (Biotinylated Human EpCAM/TROP1 Protein Tris-Bis PAGEBiotinylated Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..)
Product Categories/Family for EpCAM/TROP1 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,932 Da
NCBI Official Full Name
epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
EPCAM
NCBI Official Synonym Symbols
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
NCBI Protein Information
epithelial cell adhesion molecule; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated pro
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Gene Name
EPCAM
UniProt Synonym Gene Names
GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314
UniProt Entry Name
EPCAM_HUMAN

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Product Notes

The EpCAM/TROP1 epcam (Catalog #AAA76226) is a Recombinant Protein produced from HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EpCAM/TROP1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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