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IP (Immunoprecipitation)
(EpCAM was immunoprecipitated using: Lane A:0.5 mg HCT116 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate 4 uL anti-EpCAM rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-EpCAM rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size :35 kDa)
Rabbit anti-Human EpCAM/TROP1 Polyclonal Antibody | anti-EpCAM antibody
Anti-EpCAM/TROP1 Antibody, Rabbit Polyclonal
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
EpCAM/TROP1, Antibody; Anti-EpCAM/TROP1 Antibody, Rabbit Polyclonal; EpCAM/TROP1 Antibody, Rabbit PAb, Antigen Affinity Purified; epithelial cell adhesion molecule; Anti-17-1A Antibody; Anti-323/A3 Antibody; Anti-Ber-Ep4 Antibody; Anti-BerEp4 Antibody; Anti-CD326 Antibody; Anti-CO-17A Antibody; Anti-DIAR5 Antibody; Anti-EGP-2 Antibody; Anti-EGP314 Antibody; Anti-EGP34 Antibody; Anti-EGP40 Antibody; Anti-Ep-CAM Antibody; Anti-EPCAM Antibody; Anti-ESA Antibody; Anti-GA733-2 Antibody; Anti-HEA125 Antibody; Anti-HNPCC8 Antibody; Anti-KS1/4 Antibody; Anti-KSA Antibody; Anti-Ly74 Antibody; Anti-LYNCH8 Antibody; Anti-M1S2 Antibody; Anti-M4S1 Antibody; Anti-MH99 Antibody; Anti-MIC18 Antibody; Anti-MK-1 Antibody; Anti-MOC-31 Antibody; Anti-MOC31 Antibody; Anti-TACST-1 Antibody; Anti-TACSTD1 Antibody; Anti-TROP1 Antibody; anti-EpCAM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human EpCAM/TROP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-EpCAM antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant Human EpCAM/TROP1 Protein ( NP_002345.1; Met1-Lys265). EpCAM/TROP1 specific IgG was purified by Human EpCAM/TROP1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
IP (Immunoprecipitation)
(EpCAM was immunoprecipitated using: Lane A:0.5 mg HCT116 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate 4 uL anti-EpCAM rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-EpCAM rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size :35 kDa)
IP (Immunoprecipitation)
(EpCAM was immunoprecipitated using: Lane A:0.5 mg HCT116 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate 4 uL anti-EpCAM rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-EpCAM rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size :35 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of EpCAM-His in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human EpCAM-His polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of EpCAM-His in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human EpCAM-His polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human EpCAM in human carcinoma of sigmoid with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human EpCAM in human carcinoma of sigmoid with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-EpCAM rabbit polyclonal antibody at 1:500 dilutionLane A: HCT116 Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:37 kDa)
WB (Western Blot)
(Anti-EpCAM rabbit polyclonal antibody at 1:500 dilutionLane A: HCT116 Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:37 kDa)
Related Product Information for anti-EpCAM antibody
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type I transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
References
Brunne. A. e. al. (2008. EpCA. i. predominantl. expresse. i. hig. grad. an. advance. stag. urothelia. carcinom. o. th. bladder. . Cli. Pathol. 61(3):307-10.
Trzpi. M. e. al. (2008. EpCA. i. morphogenesis. Fron. Biosci. 13. 5050-5.
Mun. M. e. al. (2009. Th. emergin. rol. o. EpCA. i. cance. an. ste. cel. signaling. Cance. Res. 69(14). 5627-9.
Carpente. G. e. al. (2009. EpCAM. anothe. surface-to-nucleu. missile. Cance. Cell. 15(3). 165-6.
Trzpi. M. e. al. (2008. EpCA. i. morphogenesis. Fron. Biosci. 13. 5050-5.
Mun. M. e. al. (2009. Th. emergin. rol. o. EpCA. i. cance. an. ste. cel. signaling. Cance. Res. 69(14). 5627-9.
Carpente. G. e. al. (2009. EpCAM. anothe. surface-to-nucleu. missile. Cance. Cell. 15(3). 165-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28.2kDa (248aa) 28-40kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
tumor-associated calcium signal transducer 1
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
EPCAM
NCBI Official Synonym Symbols
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
NCBI Protein Information
epithelial cell adhesion molecule
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Product Notes
The EpCAM (Catalog #AAA259414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EpCAM/TROP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EpCAM/TROP1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the EpCAM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EpCAM/TROP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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