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Application Data
(Loaded Recombinant Human Ephrin A3 Protein, hFc Tag on proA Biosensor, can bind Recombinant Cynomolgus EPHA5 Protein, His Tag (Routinely tested).)
EPHA5 recombinant protein
Recombinant Cynomolgus EPHA5 Protein (His Tag), HPLC-verified
Reactivity
Cynomolgus
Purity
>= 95% as determined by SDS-PAGE. >= 95% as determined by SEC-HPLC.
Synonyms
EPHA5; N/A; Recombinant Cynomolgus EPHA5 Protein (His Tag), HPLC-verified; Cynomolgus EPHA5 Protein (His Tag); EPHA5 recombinant protein
Host
HEK293 Cells
Reactivity
Cynomolgus
Purity/Purification
>= 95% as determined by SDS-PAGE. >= 95% as determined by SEC-HPLC.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Sequence
Ser56-Pro573
Tag
C-His
Predicted N Terminal
Ser 56
Endotoxin
< 1.0 EU perug protein as determined by the LAL method.
Protein Construction
A DNA sequence encoding the Cynomolgus EPHA5 (XP_005555256.1)(Ser56-Pro573) was expressed with a polyhistidine tag at the C-terminus.
Activity
1. Immobilized Recombinant Cynomolgus EPHA5 Protein (His Tag) at 2ug/mL (100uL/well) can bind Recombinant Human Ephrin-A3/EFNA3/EFL2 Protein (Fc Tag), the EC50 is 3-12ng/mL.
2. Loaded Recombinant Human Ephrin A3 Protein, hFc Tag on proA Biosensor, can bind Recombinant Cynomolgus EPHA5 Protein, His Tag with an affinity constant of 78.2nM as determined in BLI assay (Sartorius Octet RED384)(Routinely tested).
2. Loaded Recombinant Human Ephrin A3 Protein, hFc Tag on proA Biosensor, can bind Recombinant Cynomolgus EPHA5 Protein, His Tag with an affinity constant of 78.2nM as determined in BLI assay (Sartorius Octet RED384)(Routinely tested).
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Application Data
(Loaded Recombinant Human Ephrin A3 Protein, hFc Tag on proA Biosensor, can bind Recombinant Cynomolgus EPHA5 Protein, His Tag (Routinely tested).)
Application Data
(Loaded Recombinant Human Ephrin A3 Protein, hFc Tag on proA Biosensor, can bind Recombinant Cynomolgus EPHA5 Protein, His Tag (Routinely tested).)
Application Data
(Immobilized Recombinant Cynomolgus EPHA5 Protein (His Tag)(#AAA259393) at 2ug/mL (100uL/well) can bind Recombinant Human Ephrin-A3/EFNA3/EFL2 Protein (Fc Tag), the EC50is 3-12ng/mL.)
Application Data
(Immobilized Recombinant Cynomolgus EPHA5 Protein (His Tag)(#AAA259393) at 2ug/mL (100uL/well) can bind Recombinant Human Ephrin-A3/EFNA3/EFL2 Protein (Fc Tag), the EC50is 3-12ng/mL.)
Application Data
Application Data
Application Data
Application Data
NCBI and Uniprot Product Information
Molecular Weight
The recombinant Cynomolgus EPHA5 consists of 529 amino acids and predicts a molecular mass of 59.01 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 76 kDa protein in SDS-PAGE under reducing conditions.
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Product Notes
The EPHA5 (Catalog #AAA259393) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Cynomolgus EPHA5 Protein (His Tag), HPLC-verified reacts with Cynomolgus and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: Ser56-Pro5 73. It is sometimes possible for the material contained within the vial of "EPHA5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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