Rabbit EPHA5 Polyclonal Antibody | anti-EPHA5 antibody

Phospho-EPHA5 (Tyr650) Antibody

Cat. Num. AAA329905

• Gene Names: EPHA5; CEK7; EHK1; HEK7; TYRO4
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
• Applications: ELISA, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: EPHA5, Antibody; Phospho-EPHA5 (Tyr650) Antibody; Brain-specific kinase; bsk; CEK 7; Eck like sequence 1; EHK 1; EHK-1; EK7; Els 1; EpHhomology kinase 1; EPH-like kinase 7; EPHA5; EPHA5_HUMAN; Ephrin type A receptor 5; Ephrin type-A receptor 5; HEK 7; hEK7; Receptor protein tyrosine kinase HEK 7; Rek 7; TYRO 4; Tyrosine protein kinase receptor EHK 1; anti-EPHA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-EPHA5 (Tyr650) Antibody detects endogenous levels of EPHA5 only when phosphorylated at Tyr650.; Tissue Specificity: Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-EPHA5 antibody: ELISA (Peptide ELISA), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human EPHA5 around the phosphorylation site of Tyr650.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5. Dephosphorylation upon stimulation by glucose, inhibits EPHA5 forward signaling and results in insulin secretion (By similarity).
• Subunit Structure: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts (via SAM domain) with SAMD5 (via SAM domain) (By similarity).
• Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
• Subcellular Location: Cell membrane>Single-pass type I membrane protein. Cell projection>Axon. Cell projection>Dendrite.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of EPHA5 (Phospho-Tyr650) using PMA treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot)

(Western blot analysis of extracts from various samples, using Phospho-EPHA5 (Tyr650) Antibody.Lane 1: UV treated MCF7 cells, blocked with antigen-specific peptides,Lane 2: UV treated MCF7 cells,Lane 3: RAW264.7 cells LPS,Lane 4: C6 cells LPS,Lane 5: HepG2 cells lps.)

Related Product Information for anti-EPHA5 antibody

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 221625401
• NCBI GeneID: 2044
• NCBI Accession #: NP_004430.4
• NCBI GenBank Nucleotide #: NM_004439.5
• UniProt Accession #: P54756; Q7Z3F2
• Molecular Weight: 114,803 Da
• NCBI Official Full Name: ephrin type-A receptor 5 isoform a
• NCBI Official Synonym Full Names: EPH receptor A5
• NCBI Official Symbol: EPHA5
• NCBI Official Synonym Symbols: CEK7; EHK1; HEK7; TYRO4
• NCBI Protein Information: ephrin type-A receptor 5; EK7; EHK-1; EPH-like kinase 7; EPH homology kinase 1; Eph homology kinase-1; brain-specific kinase; receptor protein-tyrosine kinase HEK7; tyrosine-protein kinase receptor EHK-1
• UniProt Protein Name: Ephrin type-A receptor 5
• UniProt Gene Name: EPHA5
• UniProt Synonym Gene Names: BSK; EHK1; HEK7; TYRO4; EHK-1; EK7; hEK7
• UniProt Entry Name: EPHA5_HUMAN