IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Mouse anti-Human EPHA5 Monoclonal Antibody | anti-EPHA5 antibody
EPHA5 Antibody
Gene Names
EPHA5; EK7; CEK7; EHK1; HEK7; EHK-1; TYRO4
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
EPHA5, Antibody; EPHA5 Antibody; Ephrin type-A receptor 5; Brain-specific kinase; EPH homology kinase 1; EHK-1; EPH-like kinase 7; EK7; hEK7; EPHA5; BSK; EHK1; HEK7; TYRO4; anti-EPHA5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1κ
Clone Number
46CT61.6.4
Specificity
Purified His-tagged EPHA5 protein(Fragment) was used to produced this monoclonal antibody.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1037
Applicable Applications for anti-EPHA5 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-EPHA5 Monoclonal Antibody by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified Mab.(1:2000))
WB (Western Blot)
(Western blot analysis of anti-EPHA5 Monoclonal Antibody by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified Mab.(1:2000))
WB (Western Blot)
(Western blot analysis of lysate from U-251 MG cell line, using EphA5 Antibody. AAA283574 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from U-251 MG cell line, using EphA5 Antibody. AAA283574 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-EPHA5 antibody
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Two transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-EPHA5 antibody
References
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Clinical significance of ephrin (eph)-A1, -A2, -a4, -a5 and -a7 receptors in pancreatic ductal adenocarcinoma. Giaginis C, et al. Pathol Oncol Res, 2010 Jun. PMID 19949912.
Frequent epigenetic inactivation of the receptor tyrosine kinase EphA5 by promoter methylation in human breast cancer. Fu DY, et al. Hum Pathol, 2010 Jan. PMID 19733895.
Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival. Herath NI, et al. BMC Cancer, 2006 Jun 1. PMID 16737551.
The LIFEdb database in 2006. Mehrle A, et al. Nucleic Acids Res, 2006 Jan 1. PMID 16381901.
Clinical significance of ephrin (eph)-A1, -A2, -a4, -a5 and -a7 receptors in pancreatic ductal adenocarcinoma. Giaginis C, et al. Pathol Oncol Res, 2010 Jun. PMID 19949912.
Frequent epigenetic inactivation of the receptor tyrosine kinase EphA5 by promoter methylation in human breast cancer. Fu DY, et al. Hum Pathol, 2010 Jan. PMID 19733895.
Over-expression of Eph and ephrin genes in advanced ovarian cancer: ephrin gene expression correlates with shortened survival. Herath NI, et al. BMC Cancer, 2006 Jun 1. PMID 16737551.
The LIFEdb database in 2006. Mehrle A, et al. Nucleic Acids Res, 2006 Jan 1. PMID 16381901.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114803
NCBI Official Full Name
ephrin type-A receptor 5 isoform a
NCBI Official Synonym Full Names
EPH receptor A5
NCBI Official Symbol
EPHA5
NCBI Official Synonym Symbols
EK7; CEK7; EHK1; HEK7; EHK-1; TYRO4
NCBI Protein Information
ephrin type-A receptor 5
UniProt Protein Name
Ephrin type-A receptor 5
UniProt Gene Name
EPHA5
UniProt Synonym Gene Names
BSK; EHK1; HEK7; TYRO4; EHK-1; EK7; hEK7
UniProt Entry Name
EPHA5_HUMAN
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Product Notes
The EPHA5 epha5 (Catalog #AAA283574) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EPHA5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EPHA5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EPHA5 epha5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHA5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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