Application Data
(HTRF assay for ERBB2 (676-1255) activity 1uM TK substrate was incubated with different concentrations of ERBB2 (676-1255) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
ERBB2 recombinant protein
Recombinant ERBB2 (676-1255) protein
Synonyms
ERBB2; N/A; Recombinant ERBB2 (676-1255) protein; HER2; NEU; CD340; ERBB2 recombinant protein
Host
Baculovirus
Form/Format
Recombinant ERBB2 (676-1255) protein is supplied in 25 mM HEPESNaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant ERBB2 (676-1255) protein that includes amino acids 676-1255 of human ERBB2 protein (accession number NP_004439.2) was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag. The molecular weight of the protein is 65.33kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(HTRF assay for ERBB2 (676-1255) activity 1uM TK substrate was incubated with different concentrations of ERBB2 (676-1255) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for ERBB2 (676-1255) activity 1uM TK substrate was incubated with different concentrations of ERBB2 (676-1255) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant ERBB2 (676-1255) protein 10% SDS-PAGE Coomassie staining MW: 65.33kDa Purity: >=90%)
SDS-PAGE
(Recombinant ERBB2 (676-1255) protein 10% SDS-PAGE Coomassie staining MW: 65.33kDa Purity: >=90%)
Related Product Information for ERBB2 recombinant protein
ERBB2(Receptor tyrosine-protein kinase erbB-2) is a protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1- RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membranebound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.In the nucleus is involved in transcriptional regulation. Associates with the 5’-TCAAATTC-3’ sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Product Categories/Family for ERBB2 recombinant protein
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Product Notes
The ERBB2 (Catalog #AAA60366) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ERBB2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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