IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with ErbB2-pY1248.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit ERBB2 Polyclonal Antibody | anti-ERBB2 antibody
ERBB2 Antibody (C-term Y1248)
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ERBB2, Antibody; ERBB2 Antibody (C-term Y1248); Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; ERBB2; HER2; MLN19; NEU; NGL; anti-ERBB2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1227-1255 amino acids from the C-terminal region of human ERBB2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.32 (varies by lot)
Sequence Positions
1227-1255
Sequence Length
1225
Applicable Applications for anti-ERBB2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with ErbB2-pY1248.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with ErbB2-pY1248.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ERBB2 (arrow) in T47D cell lysates, either noninduced (Lane 1) or induced with HRG (Lane 2).)
WB (Western Blot)
(Western blot analysis of ERBB2 (arrow) in T47D cell lysates, either noninduced (Lane 1) or induced with HRG (Lane 2).)
WB (Western Blot)
(Western blot analysis of ERBB2(arrow) using rabbit polyclonal ERBB2 Antibody (Y1248). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERBB2 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of ERBB2(arrow) using rabbit polyclonal ERBB2 Antibody (Y1248). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERBB2 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-ERBB2 antibody
ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.
Product Categories/Family for anti-ERBB2 antibody
References
Ehsani, A., et al., Genomics 15(2):426-429 (1993).
Yamamoto, T., et al., Nature 319(6050):230-234 (1986).
Coussens, L., et al., Science 230(4730):1132-1139 (1985).
Semba, K., et al., Proc. Natl. Acad. Sci. U.S.A. 82(19):6497-6501 (1985).
Yamamoto, T., et al., Nature 319(6050):230-234 (1986).
Coussens, L., et al., Science 230(4730):1132-1139 (1985).
Semba, K., et al., Proc. Natl. Acad. Sci. U.S.A. 82(19):6497-6501 (1985).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
137910
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-2 isoform b
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19
UniProt Entry Name
ERBB2_HUMAN
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Product Notes
The ERBB2 erbb2 (Catalog #AAA289300) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1227-1255. The ERBB2 Antibody (C-term Y1248) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ERBB2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ERBB2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERBB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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