IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using ERBB2 Antibody at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200))
Rabbit ERBB2 Polyclonal Antibody | anti-ERBB2 antibody
ERBB2 Antibody
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
ERBB2, Antibody; ERBB2 Antibody; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN19; HER-2/neu; anti-ERBB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ERBB2 protein.
Purity/Purification
Antigen affinity purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.8mg/ml (varies by lot)
Sequence Length
1225
Applicable Applications for anti-ERBB2 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Synthetic peptide of human ERBB2
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C
IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using ERBB2 Antibody at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200))
IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using ERBB2 Antibody at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200))
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ERBB2 Antibody at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200))
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ERBB2 Antibody at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200))
SDS-PAGE
(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane 1-2: Hela and HUVEC cell lysates, Primary antibody:ERBB2 antibody at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
SDS-PAGE
(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane 1-2: Hela and HUVEC cell lysates, Primary antibody:ERBB2 antibody at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Related Product Information for anti-ERBB2 antibody
The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity. While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members. Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers. Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase. ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway.
Product Categories/Family for anti-ERBB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,382 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-2 isoform b
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19
UniProt Entry Name
ERBB2_HUMAN
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Product Notes
The ERBB2 erbb2 (Catalog #AAA309342) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERBB2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERBB2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ERBB2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERBB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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