SDS-PAGE
D-glucuronyl C5-epimerase (GLCE) Recombinant Protein | GLCE recombinant protein
Recombinant Human D-glucuronyl C5-epimerase (GLCE), partial
Gene Names
GLCE; HSEPI
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
D-glucuronyl C5-epimerase (GLCE); N/A; Recombinant Human D-glucuronyl C5-epimerase (GLCE), partial; Heparan sulfate C5-epimerase; Hsepi; Heparin/heparan sulfate:glucuronic acid C5-epimerase; Heparosan-N-sulfate-glucuronate 5-epimerase; GLCE recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
29-617aa; Partial
Sequence
NKCSSDKAIQFPRRSSSGFRVDGFEKRAAASESNNYMNHVAKQQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLSTQWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDKNKPNDWTVPKGCFMANVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKFLTNGSVSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTVTRDLVTDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAFFAASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVRAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTGSGTIYDLRHFMLGIAPNLARWDYHTTHINQLQLLSTIDESPVFKEFVKRWKSYLKGSRAKHN
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for GLCE recombinant protein
Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.
Product Categories/Family for GLCE recombinant protein
References
Homo sapiens D-glucuronyl C5-epimerase (GLCE) .Ghiselli G.Analysis of the DNA sequence and duplication history of human chromosome 15.Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.Nature 440:671-675(2006) Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.DNA Res. 5:355-364(1998) Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.J. Proteome Res. 8:651-661(2009) Using engineered 2-O-sulfotransferase to determine the activity of heparan sulfate C5-epimerase and its mutants.Li K., Bethea H.N., Liu J.J. Biol. Chem. 285:11106-11113(2010) Uncovering a biphasic catalytic mode of C5-epimerase in heparan sulfate biosynthesis.Sheng J., Xu Y., Dulaney S.B., Huang X., Liu J.J. Biol. Chem. 287:20996-21002(2012)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70.9 kDa
NCBI Official Full Name
D-glucuronyl C5-epimerase
NCBI Official Synonym Full Names
glucuronic acid epimerase
NCBI Official Symbol
GLCE
NCBI Official Synonym Symbols
HSEPI
NCBI Protein Information
D-glucuronyl C5-epimerase
UniProt Protein Name
D-glucuronyl C5-epimerase
UniProt Gene Name
GLCE
UniProt Synonym Gene Names
; Hsepi
UniProt Entry Name
GLCE_HUMAN
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Product Notes
The GLCE glce (Catalog #AAA115339) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 29-617aa; Partial. The amino acid sequence is listed below: NKCSSDKAIQ FPRRSSSGFR VDGFEKRAAA SESNNYMNHV AKQQSEEAFP QEQQKAPPVV GGFNSNVGSK VLGLKYEEID CLINDEHTIK GRREGNEVFL PFTWVEKYFD VYGKVVQYDG YDRFEFSHSY SKVYAQRAPY HPDGVFMSFE GYNVEVRDRV KCISGVEGVP LSTQWGPQGY FYPIQIAQYG LSHYSKNLTE KPPHIEVYET AEDRDKNKPN DWTVPKGCFM ANVADKSRFT NVKQFIAPET SEGVSLQLGN TKDFIISFDL KFLTNGSVSV VLETTEKNQL FTIHYVSNAQ LIAFKERDIY YGIGPRTSWS TVTRDLVTDL RKGVGLSNTK AVKPTKIMPK KVVRLIAKGK GFLDNITIST TAHMAAFFAA SDWLVRNQDE KGGWPIMVTR KLGEGFKSLE PGWYSAMAQG QAISTLVRAY LLTKDHIFLN SALRATAPYK FLSEQHGVKA VFMNKHDWYE EYPTTPSSFV LNGFMYSLIG LYDLKETAGE KLGKEARSLY ERGMESLKAM LPLYDTGSGT IYDLRHFMLG IAPNLARWDY HTTHINQLQL LSTIDESPVF KEFVKRWKSY LKGSRAKHN. It is sometimes possible for the material contained within the vial of "D-glucuronyl C5-epimerase (GLCE), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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