FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black li)
Goat RET Antibody | anti-RET antibody
Goat Anti-RET Antibody
Gene Names
RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
Reactivity
HumanExpected from sequence similarity: Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RET; N/A; Goat Anti-RET Antibody; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease) ; HGNC:9967; CDHF12; HSCR1; MEN2A; MEN2B; MTC1; PTC; RET-ELE1; RET51 ; RET transforming sequence; cadherin family member 12; fusion gene; hydroxyaryl-protein kinase; oncogene RET; proto-oncogene; receptor tyrosine kinase; ret proto-oncogene; anti-RET antibody
Host
Goat
Reactivity
Human
Expected from sequence similarity: Human
Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
1114
Applicable Applications for anti-RET antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence NDTKALRRPKCAELH, from the internal region of the protein sequence according to NP_066124.1; NP_065681.1; NP_001342145.1.
Peptide Sequence
NDTKALRRPKCAELH
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black li)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black li)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)
References
Gartner W, Mineva I, Daneva T, Baumgartner-Parzer S, Niederle B, Vierhapper H, Weissel M, Wagner L.: A newly identified RET proto-oncogene polymorphism is found in a high number of endocrine tumor patients. Hum Genet. 2005 Apr 20. PMID: 15841388
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase receptor Ret isoform a
NCBI Official Synonym Full Names
ret proto-oncogene
NCBI Official Symbol
RET
NCBI Official Synonym Symbols
PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
NCBI Protein Information
proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Gene Name
RET
UniProt Synonym Gene Names
CDHF12; CDHR16; PTC; RET51
UniProt Entry Name
RET_HUMAN
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Product Notes
The RET ret (Catalog #AAA61825) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-RET Antibody reacts with Human Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's RET can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the RET ret for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RET, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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