IF (Immunofluorescence)
(Confocal immunofluorescent analysis of RET Antibody (Ascites) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Mouse anti-Human RET Monoclonal Antibody | anti-RET antibody
RET Antibody (Ascites)
Gene Names
RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Synonyms
RET, Antibody; RET Antibody (Ascites); Proto-oncogene tyrosine-protein kinase receptor Ret; Cadherin family member 12; Proto-oncogene c-Ret; Soluble RET kinase fragment; Extracellular cell-membrane anchored RET cadherin 120 kDa fragment; RET; CDHF12; CDHR16; PTC; RET51; anti-RET antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM,K
Clone Number
188CT11.2.3
Specificity
This RET Monoclonal antibody was raised using purified His-tagged recombinant human RET.
Form/Format
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Sequence Length
1072
Applicable Applications for anti-RET antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of RET Antibody (Ascites) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of RET Antibody (Ascites) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(RET Monoclonal(Ascites) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal(Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(RET Monoclonal(Ascites) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal(Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(RET western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RET antibody detected the RET protein (arrow).)
WB (Western Blot)
(RET western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RET antibody detected the RET protein (arrow).)
Related Product Information for anti-RET antibody
This gene, a member of the cadherin superfamily, encodes
one of the receptor tyrosine kinases, which are cell-surface
molecules that transduce signals for cell growth and
differentiation. This gene plays a crucial role in neural crest
development, and it can undergo oncogenic activation in vivo and in
vitro by cytogenetic rearrangement. Mutations in this gene are
associated with the disorders multiple endocrine neoplasia, type
IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease,
and medullary thyroid carcinoma. Two transcript variants encoding
different isoforms have been found for this gene. Additional
transcript variants have been described but their biological
validity has not been confirmed.
one of the receptor tyrosine kinases, which are cell-surface
molecules that transduce signals for cell growth and
differentiation. This gene plays a crucial role in neural crest
development, and it can undergo oncogenic activation in vivo and in
vitro by cytogenetic rearrangement. Mutations in this gene are
associated with the disorders multiple endocrine neoplasia, type
IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease,
and medullary thyroid carcinoma. Two transcript variants encoding
different isoforms have been found for this gene. Additional
transcript variants have been described but their biological
validity has not been confirmed.
Product Categories/Family for anti-RET antibody
References
Siqueira, D.R., et al. Endocr. Relat. Cancer 17(4):953-963(2010)
Gockel, H.R., et al. Hum. Genet. 128(4):353-364(2010)
Kim, H.K., et al. Anticancer Res. 30(9):3621-3627(2010)
Pacini, F., et al. Clin Oncol (R Coll Radiol) 22(6):475-485(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Gockel, H.R., et al. Hum. Genet. 128(4):353-364(2010)
Kim, H.K., et al. Anticancer Res. 30(9):3621-3627(2010)
Pacini, F., et al. Clin Oncol (R Coll Radiol) 22(6):475-485(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
124319
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase receptor Ret isoform c
NCBI Official Synonym Full Names
ret proto-oncogene
NCBI Official Symbol
RET
NCBI Official Synonym Symbols
PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
NCBI Protein Information
proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Gene Name
RET
UniProt Synonym Gene Names
CDHF12; CDHR16; PTC; RET51
UniProt Entry Name
RET_HUMAN
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Product Notes
The RET ret (Catalog #AAA283414) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RET Antibody (Ascites) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RET can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RET ret for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RET, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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