anti-Human RET Polyclonal Antibody | anti-RET antibody

Rabbit Polyclonal to Human RET

Cat. Num. AAA51701

• Gene Names: RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Ammonium sulfate precipitation
• Synonyms: RET, Antibody; Rabbit Polyclonal to Human RET; Anti-RET Antibody (C-Terminus) IHC-plus; RET; Cadherin family member 12; CDHF12; C-ret; Hirschsprung disease 1; HSCR1; Hydroxyaryl-protein kinase; Oncogene ret; Proto-oncogene c-Ret; RET-ELE1; RET51; Ret proto-oncogene; RET transforming sequence; RET43; Men2-ret; MEN2B; MTC1; Human RET; anti-RET antibody

• Host: RET antibody was produced in Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The RET antibody is generated from rabbits immunized with KLH cinjugated synthetic peptide between 1012-1042 amino acids from the C-Terminal region of human RET.
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Positions: 1012-1042

• Applicable Applications for anti-RET antibody: WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Target: RET
• Family: Protein Kinase / cadherin
• Immunogen Species: RET antibody was raised against Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term: 4°C; Long Term: -20°C; Avoid freeze-thaw cycles.

WB (Western Blot)

(Western blot of RET antibody on 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RET gene.)

WB (Western Blot)

(Western blot analysis of RET (arrow) using rabbit polyclonal RET Antibody (C-term L1027). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RET gene (Lane 2) (Origene Technologies).)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

IHC (Immunohistochemistry)

(Anti-RET antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-RET antibody

The recombination of RET tyrosine kinase with several genes, such as GOLGA5 and the H4 gene, results in a consitutively active oncogenic protein. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma.

Product Categories/Family for anti-RET antibody

Family: Protein Kinase; Subfamily: Cadherin

NCBI and Uniprot Product Information

• NCBI GI #: 10862701
• NCBI GeneID: 5979
• NCBI Accession #: NP_065681.1
• NCBI GenBank Nucleotide #: NM_020630.4
• UniProt Accession #: P07949; Q15250; Q9BTB0; Q9H4A2; A8K6Z2
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase receptor Ret isoform c
• NCBI Official Synonym Full Names: ret proto-oncogene
• NCBI Official Symbol: RET
• NCBI Official Synonym Symbols: PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase receptor Ret; proto-oncogene c-Ret; receptor tyrosine kinase; RET transforming sequence; cadherin family member 12; hydroxyaryl-protein kinase; RET receptor tyrosine kinase; rearranged during transfection; cadherin-related family member 16; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase receptor Ret
• UniProt Gene Name: RET
• UniProt Synonym Gene Names: CDHF12; CDHR16; PTC; RET51
• UniProt Entry Name: RET_HUMAN