<
IHC (Immunohistochemistry)
((2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat Pituitary Tumor-Transforming 1/Securin Antibody | anti-PTTG1 antibody
Goat Anti-Pituitary Tumor-Transforming 1/Securin Antibody
Gene Names
PTTG1; EAP1; PTTG; HPTTG; TUTR1
Reactivity
HumanExpected from sequence similarity: Human, Mouse, Dog
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Pituitary Tumor-Transforming 1/Securin; N/A; Goat Anti-Pituitary Tumor-Transforming 1/Securin Antibody; PTTG1; pituitary tumor-transforming 1 ; HGNC:9690; EAP1; HPTTG; PTTG; SECURIN; TUTR1; ESP1-associated protein 1; pituitary tumor-transforming protein 1; tumor-transforming protein 1; anti-PTTG1 antibody
Host
Goat
Reactivity
Human
Expected from sequence similarity: Human, Mouse, Dog
Expected from sequence similarity: Human, Mouse, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
202
Applicable Applications for anti-PTTG1 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-ILDEERELEKLFQ, from the internal region of the protein sequence according to NP_004210.1.
Peptide Sequence
ILDEERELEKLFQ
Epitope
Internal region
Important comments
Likely to cross-react to PTTG2 and PTTG3
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IHC (Immunohistochemistry)
((2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)
References
Park KH, Choi SE, Eom M, Kang Y.: Downregulation of the anaphase-promoting complex (APC)7 in invasive ductal carcinomas of the breast and its clinicopathologic relationships. Breast Cancer Res. 2005;7(2):R238-47. Epub 2005 Jan 14. PMID: 15743504
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
securin
NCBI Official Synonym Full Names
PTTG1 regulator of sister chromatid separation, securin
NCBI Official Symbol
PTTG1
NCBI Official Synonym Symbols
EAP1; PTTG; HPTTG; TUTR1
NCBI Protein Information
securin
UniProt Protein Name
Securin
UniProt Gene Name
PTTG1
UniProt Synonym Gene Names
EAP1; PTTG; TUTR1; Tumor-transforming protein 1; hPTTG
UniProt Entry Name
PTTG1_HUMAN
Similar Products
Product Notes
The PTTG1 pttg1 (Catalog #AAA61821) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Pituitary Tumor-Transforming 1/Securin Antibody reacts with Human Expected from sequence similarity: Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Pituitary Tumor-Transforming 1/Securin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the PTTG1 pttg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pituitary Tumor-Transforming 1/Securin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.