GSK3 beta ELISA Kit | GSK3B elisa kit

GSK3 beta (Phospho-Ser9) Phospho Sandwich ELISA Kit

Cat. Num. AAA318624

• Reactivity: Human, Mouse, Rat
• Synonyms: GSK3 beta; N/A; GSK3 beta (Phospho-Ser9) Phospho Sandwich ELISA Kit; Factor A; GSK-3 alpha; GSK3A; Glycogen synthase kinase-3 alpha; kinase GSK3-alpha; GSK3B elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The GSK3 beta (Phospho-Ser9) Phospho Sandwich ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can detect GSK3 beta protein phosphorylation and expression profile in cell lysates. The kit can be used for measuring the relative amounts of phosphorylated GSK3 beta in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on GSK3 beta phosphorylation.
• Sequence Length: 420
• Assay Type: Quantitative Sandwich

• Preparation and Storage: Store entire kit at 4 degree C until use. Kit expiration is 3 months from date of shipment.

ELISA

(Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-GSK3 beta (Phospho-Ser9) Antibody.)

Application Data

(HeLa cells were grown to 90% confluency and were stimulated with EGF 200 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of GSK3 beta P-Ser9 versus untreated HeLa lysates.)

Related Product Information for GSK3B elisa kit

Principle of the Assay: The Phospho Sandwich GSK3 beta (Phospho-Ser9) ELISA Kit contains the components necessary for semi-quantitative determination of GSK3 beta (Phospho-Ser9) concentrations within experimental cell lysate samples. This particular immunoassay utilizes the traditional "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope GSK3 beta (Phospho-Ser9) while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer where data analysis may begin.

Product Categories/Family for GSK3B elisa kit

Phospho Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 225903437
• NCBI GeneID: 2932
• NCBI Accession #: NP_001139628.1
• NCBI GenBank Nucleotide #: NP_001139628.1
• UniProt Accession #: P49841
• NCBI Official Full Name: glycogen synthase kinase-3 beta isoform 2
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_HUMAN