WB (Western Blot)
(Anti-GSK3B pS9 Antibody - Western Blot. Western blot of Rabbit-anti-GSK3B pS9 antibody at a 1:1000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kD. Cells were serum starved for 24 h prior to extraction. Key: Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20, lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20. Molecular weight markers confirm a MW of ~49 kD. Use a 1:2000 dilution of HRP Goat-a-Rabbit IgG for detection.)
Rabbit anti-Human GSK3B / GSK3 Beta Polyclonal Antibody | anti-GSK3B antibody
Rabbit Polyclonal to Human GSK3B / GSK3 Beta
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
GSK3B / GSK3 Beta, Antibody; Rabbit Polyclonal to Human GSK3B / GSK3 Beta; Anti-GSK3B / GSK3 Beta Antibody (phospho-Ser9) IHC-plus; GSK3B; GSK-3 beta; GSK3 beta; GSK3beta; GSK3beta isoform; TPKI; GSK 3beta; Tau protein kinase I; ZGSK-3beta; Human GSK3B; GSK3 Beta; anti-GSK3B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The aa 4-12 of human GSK3 beta.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
433
Applicable Applications for anti-GSK3B antibody
ELISA, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human GSK3B / GSK3 Beta.
Immunogen Type
Synthetic peptide
Immunogen
GSK3B / GSK3 Beta antibody was raised against synthetic peptide from human GSK3B / GSK3 Beta.
Antigen Modification
pSer9
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
WB (Western Blot)
(Anti-GSK3B pS9 Antibody - Western Blot. Western blot of Rabbit-anti-GSK3B pS9 antibody at a 1:1000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kD. Cells were serum starved for 24 h prior to extraction. Key: Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20, lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20. Molecular weight markers confirm a MW of ~49 kD. Use a 1:2000 dilution of HRP Goat-a-Rabbit IgG for detection.)
WB (Western Blot)
(Anti-GSK3B pS9 Antibody - Western Blot. Western blot of Rabbit-anti-GSK3B pS9 antibody at a 1:1000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kD. Cells were serum starved for 24 h prior to extraction. Key: Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20, lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20. Molecular weight markers confirm a MW of ~49 kD. Use a 1:2000 dilution of HRP Goat-a-Rabbit IgG for detection.)
IHC (Immunohistochemistry)
(Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Related Product Information for anti-GSK3B antibody
Glycogen Synthase Kinase 3 beta is a GSK-3 multisubstrate serine/threonine kinase that is associated with the canonical Wnt signaling pathway, which is involved in mammalian brain regionalization. Glycogen Synthase Kinase 3 beta has been implicated in Alzheimer's Disease, when improper phosphorylation of the microtubule-associated protein Tau results in the accumulation of neurofibrillary tangles. Two alternatively spliced isoforms have been observed.
Product Categories/Family for anti-GSK3B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,034 Da
NCBI Official Full Name
glycogen synthase kinase-3 beta isoform 1
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
GSK3B
NCBI Protein Information
glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B
UniProt Protein Name
Glycogen synthase kinase-3 beta
UniProt Gene Name
GSK3B
UniProt Synonym Gene Names
GSK-3 beta
UniProt Entry Name
GSK3B_HUMAN
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Product Notes
The GSK3B gsk3b (Catalog #AAA50721) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human GSK3B / GSK3 Beta reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3B / GSK3 Beta can be used in a range of immunoassay formats including, but not limited to, ELISA, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the GSK3B gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3B / GSK3 Beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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