Application Data
(Recombinant Mononucleosomes (H2A.X) DNA gel. Recombinant Mononucleosomes (H2A.X) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact nucleosomes. Intact nucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wrap histone octamers to form nucleosomes.)
Mononucleosomes Recombinant Protein | H2AFX recombinant protein
Recombinant Mononucleosomes (H2A.X)
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Synonyms
Mononucleosomes; N/A; Recombinant Mononucleosomes (H2A.X); H2A Histone Family Member X; H2A/X; H2AFX recombinant protein
Host
E Coli
Form/Format
Recombinant Mononucleosomes (H2A.X)(20ug protein + 19.3ug DNA) are supplied in 10mM Tris-HCl, pH8.0, 1mM EDTA, 2mM DTT and 20% glycerol.
Protein Species
Human
Notes
Recombinant Mononucleosomes (H2A.X) are suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant Mononucleosomes (H2A.X) DNA gel. Recombinant Mononucleosomes (H2A.X) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact nucleosomes. Intact nucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wrap histone octamers to form nucleosomes.)
Application Data
(Recombinant Mononucleosomes (H2A.X) DNA gel. Recombinant Mononucleosomes (H2A.X) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact nucleosomes. Intact nucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wrap histone octamers to form nucleosomes.)
SDS-PAGE
(Recombinant Mononucleosomes (H2A.X) protein gel. 12.5% SDS-PAGE Coomassie staining Purity: ? 90%)
SDS-PAGE
(Recombinant Mononucleosomes (H2A.X) protein gel. 12.5% SDS-PAGE Coomassie staining Purity: ? 90%)
Related Product Information for H2AFX recombinant protein
Short Description: Recombinant Mononucleosomes (H2A.X) consist of a 167 bp of 601 DNA and two molecules each of histones H2A.X that includes amino acids 1-143 (end)(accession number NP_002096.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3 that includes amino acids 1-136 (end)(accession number NP_003520.1), and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). All of these histones were expressed in E Coli. The molecular weight of histone octamer is ~111.7 kDa. Nucleosomes comprise the smallest subunit of chromatin; they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
Background: In vivo, the nucleosome is the basic structural unit of chromatin. It is comprised of about 146 bp of DNA wrapped around a core of eight histones of four different types: H2A, H2B, H3 and H4. Histones are subject to posttranslational modifications, such as methylation, acetylation, phosphorylation, mono-ubiquitination, etc. Histone modifications influence multiple chromatin templated processes such as gene transcription, DNA repair and recombination. Besides the "major" histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants are involved in multiple biology processes including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing. H2A.X (also known as H2AFX, Histone Family Member X) is a histone H2A family member. In mammals, variant histone H2A.X represents 10% of the total H2A, whereas in yeast 90% of H2A is of the H2A.X class. The main function of H2AX is associated with the DNA damage repair (DDR) system which is induced by DNA double strand breaks (DSBs). In many species, including S. cerevisiae, Xenopus laevis, and Drosophila melanogaster, the fourth serine residue from the carboxyl-terminal end of histone H2A becomes phosphorylated. In mammals, this event is related to phosphorylation of the H2A.X variant histone on serine 139 by DNA-dependent protein kinases (ATR/ATM/DNA- PK). The phosphorylated form of H2A.X is commonly denoted as g-H2A.X. This H2A.XS139 phosphorylation facilitates the recruitment of the DNA damage repair machinery, as well as chromatin remodelers such as INO80 and SWR1. H2A.X is a regulator of the epithelial-mesenchymal transition.
Background: In vivo, the nucleosome is the basic structural unit of chromatin. It is comprised of about 146 bp of DNA wrapped around a core of eight histones of four different types: H2A, H2B, H3 and H4. Histones are subject to posttranslational modifications, such as methylation, acetylation, phosphorylation, mono-ubiquitination, etc. Histone modifications influence multiple chromatin templated processes such as gene transcription, DNA repair and recombination. Besides the "major" histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants are involved in multiple biology processes including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing. H2A.X (also known as H2AFX, Histone Family Member X) is a histone H2A family member. In mammals, variant histone H2A.X represents 10% of the total H2A, whereas in yeast 90% of H2A is of the H2A.X class. The main function of H2AX is associated with the DNA damage repair (DDR) system which is induced by DNA double strand breaks (DSBs). In many species, including S. cerevisiae, Xenopus laevis, and Drosophila melanogaster, the fourth serine residue from the carboxyl-terminal end of histone H2A becomes phosphorylated. In mammals, this event is related to phosphorylation of the H2A.X variant histone on serine 139 by DNA-dependent protein kinases (ATR/ATM/DNA- PK). The phosphorylated form of H2A.X is commonly denoted as g-H2A.X. This H2A.XS139 phosphorylation facilitates the recruitment of the DNA damage repair machinery, as well as chromatin remodelers such as INO80 and SWR1. H2A.X is a regulator of the epithelial-mesenchymal transition.
Product Categories/Family for H2AFX recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,145 Da
NCBI Official Full Name
histone H2A.x
NCBI Official Synonym Full Names
H2A histone family, member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
NCBI Protein Information
histone H2A.x; H2AX histone
UniProt Protein Name
Histone H2A.x
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
UniProt Entry Name
H2AX_HUMAN
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Product Notes
The H2AFX h2afx (Catalog #AAA60147) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mononucleosomes, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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