anti-Mouse TCR Antibody | anti-TCR antibody

Anti-TCR [H57-597], Hamster (Armenian) IgG

Cat. Num. AAA72248

• Reactivity: Mouse
• Applications: Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: TCR; N/A; Anti-TCR [H57-597], Hamster (Armenian) IgG; Anti-TCR [H57-597]; TCR-beta; T cell receptor; T-cell receptor; T cell receptor beta chain; TCRB; TRB; TCB1; Ti; anti-TCR antibody

• Host: Hamster (Armenian)
• Reactivity: Mouse
• Isotype: Hamster (Armenian) IgG, Lambda
• Clone Number: H57-597
• Specificity: mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TCR antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
• Immunogen: Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow cytometry using the Anti-TCR antibody H57-597 (AAA72248). Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody or the rabbit IgG version of H57-597 (AAA72248-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

IF (Immunofluorescence)

(Immunofluorescence staining of mouse splenocytes with anti-TCR (AAA72248) H57-597 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of H57-597 (AAA72248-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72248-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)

Related Product Information for anti-TCR antibody

In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006).

Product Categories/Family for anti-TCR antibody

Immunology; Cell Type Marker

References

Kubo et al. Characterisation of a monoclonal antibody which detects all murine alpha-beta T cell receptors J Immunol. 1989 Apr 15;142(8):2736-42. PMID:2467936

NCBI and Uniprot Product Information

• NCBI GI #: 135513
• UniProt Accession #: P01852
• Molecular Weight: 19,346 Da
• NCBI Official Full Name: T-cell receptor beta-1 chain C region
• UniProt Protein Name: T-cell receptor beta-1 chain C region