WB (Western Blot)
(Western blot using anti-TCR betaF1 antibody 38C2 . Jurkat (A) and Molt4 (B) cells lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 38C2 (AAA72423) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
anti-Human TCR betaF1 Antibody | anti-TCRbetaF1 antibody
Anti-TCR betaF1 [38C2]
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified. Protein A affinity purified
Synonyms
TCR betaF1; N/A; Anti-TCR betaF1 [38C2]; TCRbetaF1; T cell antigen receptor markers betaf1; CGMCCNO 20778; anti-TCRbetaF1 antibody
Reactivity
Human
Isotype
Rabbit IgG kappa
Clone Number
38C2
Specificity
The antibody is specific for TCRbetaF1.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TCRbetaF1 antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western blot using anti-TCR betaF1 antibody 38C2 . Jurkat (A) and Molt4 (B) cells lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 38C2 (AAA72423) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-TCR betaF1 antibody 38C2 . Jurkat (A) and Molt4 (B) cells lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 38C2 (AAA72423) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-TCR betaF1 antibody 38C2 . Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 38C2 (AAA72423) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72423, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-TCR betaF1 antibody 38C2 . Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 38C2 (AAA72423) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72423, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-TCRbetaF1 antibody
The original antibody was generated by immunizing mice with TCRbetaF1 protein.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Product Categories/Family for anti-TCRbetaF1 antibody
Similar Products
Product Notes
The TCRbetaF1 (Catalog #AAA72423) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TCR betaF1 [38C2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCR betaF1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TCRbetaF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR betaF1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.