IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-TCR antibody BMA031 . Immunofluorescence analysis of paraformaldehyde-fixed Jurkat cells on Shi-fix™ coverslips stained with the mouse IgG2b version of BMA031 (AAA72303) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72303, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human TCR Antibody | anti-TCR antibody
Anti-TCR [BMA031]
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence
Purity
Purified. Protein A affinity purified
Synonyms
TCR; N/A; Anti-TCR [BMA031]; Human alpha/beta T Cell Receptor (TCR); anti-TCR antibody
Reactivity
Human
Isotype
Mouse IgG2b kappa
Clone Number
BMA031
Specificity
This antibody reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. TCR-alphabeta is expressed by the majority of mature CD3+ T cells.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TCR antibody
Blocking, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-TCR antibody BMA031 . Immunofluorescence analysis of paraformaldehyde-fixed Jurkat cells on Shi-fix™ coverslips stained with the mouse IgG2b version of BMA031 (AAA72303) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72303, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Jurkat cells with anti-TCR antibody BMA031 . Immunofluorescence analysis of paraformaldehyde-fixed Jurkat cells on Shi-fix™ coverslips stained with the mouse IgG2b version of BMA031 (AAA72303) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72303, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-TCR antibody
The original antibody was generated by immunizing mice with human perihperal blood T-lymphocytes.
Describes the role of this antibody as a tool for analysing mechanisms of T cell activation, interaction between T-cells, B-cells, accessory cells and intermediating immune factors.
Describes the role of this antibody as a tool for analysing mechanisms of T cell activation, interaction between T-cells, B-cells, accessory cells and intermediating immune factors.
Product Categories/Family for anti-TCR antibody
References
Kurrle et al.
Behring Institute Mitteilungen (1986), (80):48-58.
Behring Institute Mitteilungen (1986), (80):48-58.
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Product Notes
The TCR (Catalog #AAA72303) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TCR [BMA031] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCR can be used in a range of immunoassay formats including, but not limited to, Blocking, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence). Researchers should empirically determine the suitability of the TCR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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