Application Data
(Figure 1. Response of ACTOneTM S1PR5 cell line & parental cell line to S1PACTOneTM S1PR5 cells and parental cells were plated overnight in 20 ul culture mediumon a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loading solution(membrane potential dye kit. After 2 hours of incubation at room temperature, two readingswere obtained prior to and 30 min after the addition of S1P. Ratios of the two readings (F/F0) are plotted inthe figure.A. Dose response curve of S1P in ACTOneTM S1PR5 cell line. EC50 = 36 nM in the presence ofPDE inhibitor Ro 20-1724 and ?–adren degree Ceptor agonist isoproterenol.B. Parental cells do not respond to S1P.)
Sphingosine-1-Phosphate Receptor 5 (S1PR5) Cell Line | S1PR5 cell line
Sphingosine-1-Phosphate Receptor 5 (S1PR5) ACTOne Stable Cell Line, HEK293
Gene Names
S1PR5; EDG8; S1P5; Edg-8; SPPR-1; SPPR-2
Synonyms
Sphingosine-1-Phosphate Receptor 5 (S1PR5); N/A; Sphingosine-1-Phosphate Receptor 5 (S1PR5) ACTOne Stable Cell Line, HEK293; Sphingosine-1-Phosphate Receptor 5 (S1PR5) ACTOneTM Stable Cell Line; S1PR5 cell line
Host
HEK-293 CNG cells
Detection
FlexStation or Microplate reader
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note
Product available only in the USA.
Preparation and Storage
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 degree C, preferably in liquid nitrogen vapor, until ready for use.
Application Data
(Figure 1. Response of ACTOneTM S1PR5 cell line & parental cell line to S1PACTOneTM S1PR5 cells and parental cells were plated overnight in 20 ul culture mediumon a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loading solution(membrane potential dye kit. After 2 hours of incubation at room temperature, two readingswere obtained prior to and 30 min after the addition of S1P. Ratios of the two readings (F/F0) are plotted inthe figure.A. Dose response curve of S1P in ACTOneTM S1PR5 cell line. EC50 = 36 nM in the presence ofPDE inhibitor Ro 20-1724 and ?–adren degree Ceptor agonist isoproterenol.B. Parental cells do not respond to S1P.)
Application Data
(Figure 1. Response of ACTOneTM S1PR5 cell line & parental cell line to S1PACTOneTM S1PR5 cells and parental cells were plated overnight in 20 ul culture mediumon a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loading solution(membrane potential dye kit. After 2 hours of incubation at room temperature, two readingswere obtained prior to and 30 min after the addition of S1P. Ratios of the two readings (F/F0) are plotted inthe figure.A. Dose response curve of S1P in ACTOneTM S1PR5 cell line. EC50 = 36 nM in the presence ofPDE inhibitor Ro 20-1724 and ?–adren degree Ceptor agonist isoproterenol.B. Parental cells do not respond to S1P.)
Application Data
Application Data
Related Product Information for S1PR5 cell line
Introduction: Introduction: S1PR5 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P).S1PR5 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR5-5).They areexpressed in a wide variety of tissues, with each subtype exhibiting a different cell specificity, although they are found attheir highest density on leuk degree Cytes. S1PR5 may play a regulatory role in the transformation of radial glial cells intoastr degree Cytes and may affect proliferative activity of these cells.
Description: Human S1PR5 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human S1PR5. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levelsand consequently result in ion flux (often detectable by calcium-responsive dye) and cell membranedepolarization which can be easily measured with fluorescent Membrane Potential Dye. The assayallows both end-point and kinetic measurement of intracellular cAMP changes with a FDSS, FLIPR, or a fluorescencemicroplate reader.
This cell line is a HEK-293-CNG cell line that expresses a recombinant human S1PR5. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.
Description: Human S1PR5 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human S1PR5. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levelsand consequently result in ion flux (often detectable by calcium-responsive dye) and cell membranedepolarization which can be easily measured with fluorescent Membrane Potential Dye. The assayallows both end-point and kinetic measurement of intracellular cAMP changes with a FDSS, FLIPR, or a fluorescencemicroplate reader.
This cell line is a HEK-293-CNG cell line that expresses a recombinant human S1PR5. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.
Product Categories/Family for S1PR5 cell line
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,279 Da
NCBI Official Full Name
sphingosine 1-phosphate receptor 5
NCBI Official Synonym Full Names
sphingosine-1-phosphate receptor 5
NCBI Official Symbol
S1PR5
NCBI Official Synonym Symbols
EDG8; S1P5; Edg-8; SPPR-1; SPPR-2
NCBI Protein Information
sphingosine 1-phosphate receptor 5
UniProt Protein Name
Sphingosine 1-phosphate receptor 5
UniProt Gene Name
S1PR5
UniProt Synonym Gene Names
EDG8; S1P receptor 5; S1P5; S1P receptor Edg-8
UniProt Entry Name
S1PR5_HUMAN
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Product Notes
The S1PR5 s1pr5 (Catalog #AAA62097) is a Cell Line produced from HEK-293 CNG cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Sphingosine-1-Phosphate Receptor 5 (S1PR5), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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