PlGF-1/PGF active protein

Recombinant human PlGF-1/PGF protein

Cat. Num. AAA48993

• Gene Names: PGF; PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PlGF-1/PGF; N/A; Recombinant human PlGF-1/PGF protein; Placenta growth factor; PlGF; PGF; PGFL; PLGF; placenta growth factor isoform 2; PlGF-1/PGF active protein

• Host: HEK293
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 19-149aa
• Sequence: Leu 19; LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERCGDAVPR R

• Applicable Applications for PlGF-1/PGF active protein: Bioactivity, SDS-PAGE
• Species: Human
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Related Product Information for PlGF-1/PGF active protein

Placenta growth factor (PlGF/PGF) is a member of the PDGF/VEGF-sub-family-a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. There are three isoforms of this protein: PlGF-1, PlGF-2, PlGF-3. PlGF-2 is only found in early placenta up until the 8th week of development, while PlGF-1 is specifically found in the colon as well as mammary carcinomas. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. Also, PlGF is ultimately associated with angiogenesis. These activities facilitate wound, bone fracture, and cardiac repair, but also contribute to inflammation in active sickle cell disease and atherosclerosis. Recombinant human PlGF-1/PGF, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for PlGF-1/PGF active protein

Angiogenesis, Cardiovascular; Proteins

References

Hauser, S. and H.A. Weich (1993) Growth Factors. 9:259-268.
Maglione, D., et al, (1993) Oncogene. 8:925-931.
Roncal, C., et al, (2010) Cardiovasc. Res. 86:29-36.

NCBI and Uniprot Product Information

• NCBI GI #: 333033785
• NCBI GeneID: 5228
• NCBI Accession #: NP_001193941.1
• NCBI GenBank Nucleotide #: NM_001207012.1
• UniProt Accession #: P49763; Q07101; Q9BV78; Q9Y6S8
• Molecular Weight: 24,789 Da
• NCBI Official Full Name: placenta growth factor isoform 2
• NCBI Official Synonym Full Names: placental growth factor
• NCBI Official Symbol: PGF
• NCBI Official Synonym Symbols: PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
• NCBI Protein Information: placenta growth factor; placental growth factor, vascular endothelial growth factor-related protein
• UniProt Protein Name: Placenta growth factor
• UniProt Gene Name: PGF
• UniProt Synonym Gene Names: PGFL; PLGF; PlGF
• UniProt Entry Name: PLGF_HUMAN