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product-image-AAA282599_AD11.jpg Application Data (Active Recombinant Mouse IL-12(#AAA282599)induces the mouse T cells differentiate into Th1 cells. Flow cytometry was used to detect the expression of IFN-gamma. Result showed that Th1 cells were induced successfully.(Customer Feedback Data).)

IL-12A&IL-12B active protein

Active Recombinant Mouse IL-12A&IL-12B Protein

Synonyms
IL-12A&IL-12B; N/A; Active Recombinant Mouse IL-12A&IL-12B Protein; p35; Ll12a; Il-12a; IL-12p35&p40; Il-12b; Il12p40; Il-12p40; IL-12&Il12b; IL-12 p70;?IL12A&IL12B;?; IL-12A&IL-12B active protein
Ordering
Host
HEK293 cells
Form/Format
Liquid
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Species
Mouse
Tag
C-His(IL-12)&No tag(IL-12b)
Bioactivity
1. Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-12A&IL-12B at 5 ug/mL (100 uL/well) can bind Human IL12RB1 with a linear range of 0.001-3.3 ng/mL.
2.Active Recombinant Mouse IL-12 induces the mouse T cells differentiate into Th1 cells. Flow cytometry was used to detect the expression of IFN-gamma. Result showed that Th1 cells were induced successfully..
Preparation and Storage
Store the lyophilized protein at -20 to -80 degree C for 12 months. After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid repeated freeze/thaw cycles.
Ship: Ice Pack

Application Data

(Active Recombinant Mouse IL-12(#AAA282599)induces the mouse T cells differentiate into Th1 cells. Flow cytometry was used to detect the expression of IFN-gamma. Result showed that Th1 cells were induced successfully.(Customer Feedback Data).)

product-image-AAA282599_AD11.jpg Application Data (Active Recombinant Mouse IL-12(#AAA282599)induces the mouse T cells differentiate into Th1 cells. Flow cytometry was used to detect the expression of IFN-gamma. Result showed that Th1 cells were induced successfully.(Customer Feedback Data).)

Application Data

(Immobilized Mouse IL-12A&IL-12B (#AAA282599) at 5 ug/mL (100 uL/well) can bind Human IL12RB1 with a linear range of 0.001-3.3 ng/mL.)

product-image-AAA282599_AD13.jpg Application Data (Immobilized Mouse IL-12A&IL-12B (#AAA282599) at 5 ug/mL (100 uL/well) can bind Human IL12RB1 with a linear range of 0.001-3.3 ng/mL.)

SDS-PAGE

(Recombinant Mouse IL-12A&IL-12B Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) kDa.)

product-image-AAA282599_SDS_PAGE15.jpg SDS-PAGE (Recombinant Mouse IL-12A&IL-12B Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) kDa.)
Related Product Information for IL-12A&IL-12B active protein
Active Recombinant Mouse IL-12&Il12b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala28-Arg193) of mouse IL-12A&IL-12B (Accession #NP_000790.2) fused with and a 6×His tag at the C-terminus(IL-12).
Product Categories/Family for IL-12A&IL-12B active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,179 Da
NCBI Official Full Name
interleukin-12 subunit alpha isoform 1
UniProt Protein Name
Interleukin-12 subunit alpha
UniProt Gene Name
Il12a
UniProt Synonym Gene Names
IL-12A; CLMF p35
UniProt Entry Name
IL12A_MOUSE

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Product Notes

The IL-12A&IL-12B il12a (Catalog #AAA282599) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL-12A&IL-12B, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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