WB (Western Blot)
(Detection of human IL-12B by western blot. Samples: Human recombinant IL-12B protein (0.01 ug). Antibody: Rabbit anti-IL-12B recombinant monoclonal antibody (AAA213709 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second.)
IL-12B recombinant antibody
Rabbit anti-IL-12B Recombinant Monoclonal Antibody [BLR322M]
Gene Names
IL12B; CLMF; NKSF; CLMF2; NKSF2; IL-12B
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
IL-12B, Recombinant Antibody; Rabbit anti-IL-12B Recombinant Monoclonal Antibody [BLR322M]; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 subunit beta; NK cell stimulatory factor chain 2; IMD29; NKSF2; interleukin-12 beta chain; NKSF; CLMF; IL-12B; IL12, subunit p40; IL-12 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF2; CLMF p40; natural killer cell stimulatory factor, 40 kD subunit; IMD28; Interleukin-12 subunit beta; interleukin 12B; IL-12B recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR322M
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for IL-12B recombinant antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
residues 23-328
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human IL-12B by western blot. Samples: Human recombinant IL-12B protein (0.01 ug). Antibody: Rabbit anti-IL-12B recombinant monoclonal antibody (AAA213709 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human IL-12B by western blot. Samples: Human recombinant IL-12B protein (0.01 ug). Antibody: Rabbit anti-IL-12B recombinant monoclonal antibody (AAA213709 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human IL-12B by western blot. Samples: Whole cell lysate (50 ug) from 293F cells untransfected (-) or overexpressing IL-12A, IL-12B, IL-12A and IL-12B, or IL-23A and IL-12B. Antibody: Rabbit anti-IL-12B recombinant monoclonal antibody (AAA213709 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human IL-12B by western blot. Samples: Whole cell lysate (50 ug) from 293F cells untransfected (-) or overexpressing IL-12A, IL-12B, IL-12A and IL-12B, or IL-23A and IL-12B. Antibody: Rabbit anti-IL-12B recombinant monoclonal antibody (AAA213709 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human IL-12B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293F cells overexpressing IL12B. Antibodies: Rabbit anti-IL-12B recombinant monoclonal antibody (AAA213709 lot 1) used for IP at 6 ul per reaction. IL-12B was also immunoprecipitated by a second antibody against a different epitope of IL-12B (Antibody 2). For blotting immunoprecipitated IL-12B, AAA213709 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human IL-12B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293F cells overexpressing IL12B. Antibodies: Rabbit anti-IL-12B recombinant monoclonal antibody (AAA213709 lot 1) used for IP at 6 ul per reaction. IL-12B was also immunoprecipitated by a second antibody against a different epitope of IL-12B (Antibody 2). For blotting immunoprecipitated IL-12B, AAA213709 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for IL-12B recombinant antibody
IL-12B is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD IL-12B, and the 35 kD subunit IL-12A. IL-12 is expressed by activated macrophages that serve as an essential inducer of Th1 cells development, and has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. [provided by RefSeq, Jul 2008] [taken from NCBI Entrez Gene (Gene ID: 3593)].
Product Categories/Family for IL-12B recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
328
NCBI Official Full Name
interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
NCBI Official Symbol
IL12B
NCBI Official Synonym Symbols
CLMF; NKSF; CLMF2; NKSF2; IL-12B
NCBI Protein Information
interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor,
UniProt Protein Name
Interleukin-12 subunit beta
UniProt Gene Name
IL12B
UniProt Synonym Gene Names
NKSF2; IL-12B; CLMF p40; NKSF2
UniProt Entry Name
IL12B_HUMAN
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Product Notes
The IL-12B il12b (Catalog #AAA213709) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-IL-12B Recombinant Monoclonal Antibody [BLR322M] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-12B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the IL-12B il12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-12B, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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